1. A method for the subcellular fractionation of intestinal mucosa is described. The subcellular fractions were characterized by electron microscopy and by deternining the distributions of cytochrome oxidase and alkaline phosphatase in the subcellular fractions. 2. The subcellular distribution of peptidases hydrolysing L-leucylglycine and glycylglycine has been determined in intestinal mucosa. ...

The effect of temperature on the thermodynamic ionization constants of compounds with dipole moments greater than those of the a-amino acids has not heretofore been studied. In this paper results are given for glycylglycine and c-aminocaproic acid, two substances with similar dipole moments. Measurements are also included for aspartic acid, which has recently been studied at 25” in cells withou...

This article describes a method for improving 1H NMR spectra of aqueous samples containing paramagnetic metals by precipitation metal cations with variety counteranions. The addition hydroxide, phosphate, carbonate, and arsenate to solutions transition such as Fe2+ Mn2+ can reduce line broadening improve the ability spectrometer lock on signal deuterium. is most effective under strongly alkalin...

1. The effect of peptides containing leucine and glycine on accumulation of leucine and glycine by everted jejunal rings was studied. 2. It was shown that, on a molar basis, leucyl-leucine is a more effective inhibitor of uptake of [(14)C]leucine than is either leucylglycine or glycyl-leucine. These latter dipeptides behave alike. 3. The concentration of the dipeptides and their constituent ami...

Staphylococcus capitis EPK1 produces a glycylglycine endopeptidase, ALE-1 (M. Sugai, T. Fujiwara, T. Akiyama, M. Ohara, H. Komatsuzawa, S. Inoue, and H. Suginaka, J. Bacteriol. 179:1193-1202, 1997), which hydrolyzes interpeptide pentaglycine chains of cell wall peptidoglycan of S. aureus. Characterizations of the enzyme activity and cloning of ale-1 revealed that ALE-1 is very similar to prolys...