Clinical management of diabetes must overcome the challenge of in vivo glucose sensors exhibiting lifetimes of only a few days. Limited sensor life originates from compromised enzyme stability of the sensing enzyme. Sensing enzymes degrade in the presence of low molecular weight materials (LMWM) and hydrogen peroxide in vivo. Sensing enzymes could be made to withstand these degradative effects ...

Heavy metal contamination in water is a serious risk to the public health and other life forms on earth. Current research in nanotechnology is developing new nanosystems and nanomaterials for the fast and efficient removal of pollutants and heavy metals from water. Here, we report graphene oxide-based microbots (GOx-microbots) as active self-propelled systems for the capture, transfer, and remo...

Protein conformation associates with particular properties of proteins and relates to protein-mediated diseases. Detailed elucidation of secondary and tertiary formation, stability, and the structural and dynamic properties of proteins has been one of the main topics studied in chemistry and biology. In this work, the conformation changes in glucose oxidase (GOx) induced by the graphene oxide (...

A bilayer of the polyelectrolytes poly(dimethyldiallylammonium chloride) (PDDA) and poly(sodium 4-styrenesulfonate) (PSS) was formed on a 3-mercapto-1-propanesulfonic-acid-modified Au electrode. Subsequently, multiwall carbon nanotubes (MWCNTs) wrapped by positively charged PDDA were assembled layer-by-layer with negatively charged glucose oxidase (GOx) onto the PSS-terminated bilayer. Electroc...

Due to their unique physicochemical properties, carbon nanochips (CNCs) have been used for studies of the direct electrochemical and electrocatalytic properties of oxidoreductase. In this report, a glassy carbon electrode (GCE) was modified with CNCs, and glucose oxidase (GOx) was immobilized on the modified electrode surface. Chitosan (CS) was employed to fix the GOx/CNCs tightly to the surfac...

La Glucosa Oxidasa (GOX) se ha utilizado ampliamente como aditivo alimentario debido a sus propiedades, sin embargo, no hay literatura científica que reporten sobre efectos en la conservación de bebidas preparadas partir frutas. El objetivo esta investigación fue evaluar el potencial conservante un extracto GOX, para evitar oscurecimiento jugo manzana natural y favorecer producción peróxido hid...

Abstract A glucose amperometric biosensor has been prepared by a multilayer deposition on glassy carbon electrode of Co3Mn-CO3 layered double hydroxide, oxidase (GOx) and biopolymer (carrageenan), respectively. The original transduction step consists in the electrocatalytic oxidation H2O2, enzymatically formed, inner-layer at 0.65 V/Ag-AgCl. sensitivity detection limit, obtained with this optim...

Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring molecular content these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high-resolution NMR spectroscopy. We demonstrate operation of system by activity enzymes immobilized chemically disti...

We have proposed a novel free-standing nitrogen-doped carbon nanospheres@carbon nanofibers (NCNSs@CNFs) composite film with high processability for the investigation of the direct electron transfer (DET) of glucose oxidase (GOx) and the DET-based glucose biosensing. The composites were simply prepared by controlled thermal treatment of electrospun polypyrrole nanospheres doped polyacrylonitrile...

In cultured pulmonary artery endothelial cells and other cell types, overexpression of mt-targeted DNA repair enzymes protects against oxidant-induced mitochondrial DNA (mtDNA) damage and cell death. Whether mtDNA integrity governs functional properties of the endothelium in the intact pulmonary circulation is unknown. Accordingly, the present study used isolated, buffer-perfused rat lungs to d...