this study discusses the effect of complexes of chitosan grafted polyethylenimine(ch-pei) with plasmid dna on viability of mesenchymal stem cells(mscs) derived from human marrow. ch-pei/pdna nanoparticles were synthesized through the complex coacervation method using pires plasmid containing green fluorescent protein (gfp) gene. to confirm the complexation, samples were run through an agarose g...

A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP β-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was ...

Objective(s) Mesenchymal stem cells (MSCs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. Human mesenchymal stem cells (hMSCs) are ideal targets in cell transplantation and tissue engineering. Enhanced green fluorescent protein (EGFP) has been an important reporter gene for gene therapy. The aim of this stu...

BACKGROUND In this study, Escherichia coli cells producing red fluorescent protein of Discosoma sp. (drFP583 DsRed) were investigated with flow cytometry by using 488 nm excitation. We also studied whether green fluorescent protein (GFP) and drFP583 could be detected simultaneously from a single bacterial cell. METHODS Plasmids pDsRed and pEGFP were used for the production of drFP583 and enha...

Several bioluminescent coelenterates use a secondary fluorescent protein, the green fluorescent protein (GFP), in an energy transfer reaction to produce green light. The most studied of these proteins have been the GFPs from the jellyfish Aequorea victoria and the sea pansy Renilla reniformis. Although the proteins from these organisms are not identical, they are thought to have the same chromo...

Abbreviations: CPRG, chlorophenol red-β-D-galactopyranoside; CFP, Cyan Fluorescent Protein; CHO, Chinese Hamster Ovary cells; FRET, Fluorescence Resonance Energy Transfer; GFP, Green Fluorescent Protein; HEX, hexachloro-carboxyfluorescein; HUVEC, Human Umbilical Vein Endothelial Cells; RHD, Rel Homology Domain; TET, tetrachloro-carboxyfluorescein; β-TrCP, beta-transducin repeat-containing prote...

Abbreviations: CPRG, chlorophenol red-β-D-galactopyranoside; CFP, Cyan Fluorescent Protein; CHO, Chinese Hamster Ovary cells; FRET, Fluorescence Resonance Energy Transfer; GFP, Green Fluorescent Protein; HEX, hexachloro-carboxyfluorescein; HUVEC, Human Umbilical Vein Endothelial Cells; RHD, Rel Homology Domain; TET, tetrachloro-carboxyfluorescein; β-TrCP, beta-transducin repeat-containing prote...

We have cloned an unusual colourless green fluorescent protein (GFP)-like protein from Aequorea coerulescens (acGFPL). The A. coerulescens specimens displayed blue (not green) luminescence, and no fluorescence was detected in these medusae. Escherichia coli expressing wild-type acGFPL showed neither fluorescence nor visible coloration. Random mutagenesis generated green fluorescent mutants of a...

Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed.