The herpes simplex virus type 1 (HSV-1) tegument protein, VP22 has been reported to have the property of intercellular transport. The previous studies have shown that following expression of a fusion protein containing VP22 it spreads to every cell in a monolayer and concentrates in the nucleus. In spite of these reports, some studies have shown that VP22 trafficking and its nucleus accumulatio...

A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP β-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was ...

The cover image is based on the Article Rapid prototyping enzyme homologs to improve titer of nicotinamide mononucleotide using a strategy combining cell-free protein synthesis with split GFP by Qingyan Yuan et al., https://doi.org/10.1002/bit.28326.

Since its cloning and commercial availability, applications of green fluorescent protein (GFP) as a reporter gene have become prevalent in many aspects of science. The attributes of GFP could also be applied to the area of heterologous protein production. The work described here represents the first experiments to use GFP as a generic tool to monitor protein production in bioprocess development...

Protein aggregation is a hallmark of neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS), Alzheimer's disease (AD), Parkinson's (PD), Huntington's (HD), and so on. To detect analyze soluble or diffuse protein oligomers aggregates, fluorescence correlation spectroscopy (FCS), which can the diffusion speed brightness single particle with molecule sensitivity, has been used. Ho...

Numerous genes involved in calcification, algal endosymbiosis, and the stress response have been identified corals by large-scale gene expression analysis, but functional analysis of those is lacking. There are few experimental examples manipulation corals, such as knockdown RNA interference (RNAi). The purpose this study to establish an RNAi method for coral juveniles. As a first trial, encodi...

Tiêm mao F18 đóng vai trò quan trọng giúp Enterotoxigenic Escherichia coli (ETEC) bám vào ruột và tiết độc tố gây bệnh tiêu chảy heo con sau cai sữa (post-weaning diarrhea, PWD). Vaccine là một cách thức hữu hiệu, kinh tế khả thi phòng chống ETEC trên heo. Việc phát triển vaccine uống, tạo miễn dịch niêm mạc thông qua nhắm trúng đích bào M đang được tâm. Nhiều nghiên cứu cho thấy Hsp60 có năng ...

Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for theimprovement of low transfection efficiency of chitosan. The present study aims to test thepulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposedto aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHIg-PEI complexes for 30 min during the development of ...

The GFP folding reporter assay uses a C-terminal GFP fusion to report on the folding success of upstream fused polypeptides. The GFP folding assay is widely-used for screening protein variants with improved folding and solubility, but truncation artifacts may arise during evolution, i.e. from de novo internal ribosome entry sites. One way to reduce such artifacts would be to insert target genes...