نتایج جستجو برای: griess assay
تعداد نتایج: 222159 فیلتر نتایج به سال:
Chronic inflammation can lead to several diseases that represent the leading causes of mortality worldwide. Conventional treatment carry some risks. Therefore, research on herbal medicine are suspected having anti-inflammatory effects, such as Piper betle L., is important. This study aims investigate effect P. L. extract nitric oxide a pro-inflammatory mediator. The dried leaves were extracted ...
Background and objectives: Neuronal toxicity can be induced by oxidative stress via free radicals production. In recent years, great interest has been expressed to the traditional and herbal medicines. The purpose of this study was to elucidate the neuroprotective activity of Leontice leontopetalum methanol extract against H2O2-stimulated oxidative stress in PC12 cells...
for paper presented at the 57 American Academy of Forensic Sciences, New Orleans, Louisiana, 2005.
A high-performance ionic-sensing platform has been developed by an interdisciplinary approach, combining the classical colorimetric Griess reaction and new concepts of nanotechnology, such as plasmonic coupling of nanoparticles and surface-enhanced Raman scattering (SERS) spectroscopy. This approach exploits the advantages of combined SERS/surface-enhanced resonant Raman Scattering (SERRS) by i...
Evidence supports the involvement of nitric oxide (NO) in a Varity of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, Low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is established t...
Nitric oxide (NO) plays an important role in many inflammatory responses and is also involved in carcinogenesis. In the present study, we investigated the inhibitory effect of extracts from Humulus lupulus L. on both the production of NO and the expression of inducible NO synthase (iNOS) in mouse macrophage RAW 264.7 cells. The production of NO was induced by a combination of lipopolysaccharide...
To search for novel nitric oxide (NO) releasing anti-tumor agents, a series of novel furoxan/oridonin hybrids were designed and synthesized. Firstly, the nitrate/nitrite levels in the cell lysates were tested by a Griess assay and the results showed that these furoxan-based NO-releasing derivatives could produce high levels of NO in vitro. Then the anti-proliferative activity of these hybrids a...
The tri-iodide-based chemiluminescence assay is the most widely used methodology for the detection of S-nitrosothiols (RSNOs) in biological samples. Because of the low RSNO levels detected in a number of biological compartments using this assay, criticism has been raised that this method underestimates the true values in biological samples. This claim is based on the beliefs that (i) acidified ...
OBJECTIVE To study the mechanisms of the antitumor and immunoregulation functions of polyporus polysaccharide (PPS). METHODS The production of nitric oxide (NO), the activity and mRNA expression of inducible nitric oxide synthase (iNOS) in peritoneal macrophages of mice administered with different dose of PPS were observed by Griess reaction, fluorimetry assay and RT-PCR, respectively. RESU...
Background: Emphasis on substitutional medications with the elevated bacterial resistance to current antibiotics is pivotal. We evaluated antibacterial effect of AK2 by Minimum Bactericidal Concentration (MBC) and inhibitory (MIC) its impact macrophage responses in 17 strains pathogenic bacteria. The gene expression macrophage’s cytokines was evaluated. Accordingly, bioinformatic assessment pre...
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