Bernhard Epping, Alexander P. Hansen and Peter Martin Institut für Pflanzenernährung (330), Fruwirthstr. 20, Universität Hohenheim, 70593 Stuttgart, Bundesrepublik Deutschland Z. Naturforsch. 50 c, 543-551 (1995); received March 15/May 2, 1995 Oxygen Derivates, Phaseolus vulgaris L.. Rhizobium leguminosarum, Rhizobium tropici, Symbiotic Effectivity Nodules of Rhizobium leguminosarum bv. phaseol...

A novel, basic (isoelectric point > 10), heme peroxidase isoenzyme (TP; relative molecular weight = 34,660 +/- 10, mean +/- SE) that can account for a significant part of the ascorbate peroxidase activity in tea (Camellia sinensis) leaves has been purified to homogeneity. The ultraviolet/visible absorption spectrum is typical of heme-containing plant peroxidases, with a Soret peak at 406 nm (ep...

In plants, it has been proposed that hexacoordinate (class 1) non-symbiotic Hbs (nsHb-1) function in vivo as peroxidases. However, little is known about peroxidase activity of nsHb-1. We evaluated the peroxidase activity of rice recombinant Hb1 (a nsHb-1) by using the guaiacol/H2O2 system at pH 6.0 and compared it to that from horseradish peroxidase (HRP). Results showed that the affinity of ri...

Peroxidase activity was characterized in lettuce (Lactuca sativa L.) leaf tissue. Changes in the activity and distribution of the enzyme were examined during the development of a nonhost hypersensitive reaction (HR) induced by Pseudomonas syringae (P. s.) pv phaseolicola and in response to an hrp mutant of the bacterium. Assays of activity in tissue extracts revealed pH optima of 4.5, 6.0, 5.5 ...

a hydroponics study was carried out in order to understand the behavior of antioxidant enzymes, soluble protein content and its effect on cell death in different old leaves of durum wheat. two month after planting, the different old leaves were sampled and the activity of super oxide dismutase (sod), catalase, ascorbat peroxidase and guaiacol peroxidase were measured. furthermore, the rate of c...

Aqueous crude extracts of a series of plant wastes (agricultural, wild plants, residues from sports activities (grass), ornamental residues (gardens)) from 17 different plant species representative of the typical biodiversity of the Iberian peninsula were investigated as new sources of peroxidases (EC 1.11.1.7). Of these, lentil (Lens culinaris L.) stubble crude extract was seen to provide one ...

A procedure for the extraction and isolation of the peroxidase in pig thyroid glands has been described. The enzyme has been purified by gel filtration and diethylaminoethyl chromatography, and its spectral properties suggest that the enzyme is a typical hemoprotein peroxidase, while gel filtration experiments have shown it to have a molecular weight of 104,000. The specific activity of the pre...