This article presents the results of research on production mutant doubled haploids turnip rape (Brassica rapa L.ssp.Oleifera), as well its interspecies hybrids with rapeseed napus). An  immature embryo culture  was used to increase productivity inter-specific hybridization. Studies have obtained interspecific rapeseed. The largest number plants observed in where mother plant Furtherm...

Doubled haploid (DH) technology is a powerful tool in plant breeding to reduce the time and costs needed to produce pure lines, the cornerstone of hybrid seed production. This biotechnological alternative to classic methods allows for a reduction of the typical 7–8 inbreeding generations needed to fix a hybrid genotype to only one in vitro generation. It is therefore much faster and cheaper, be...

Much of our knowledge about the fitness effects of new mutations has been gained from mutation accumulation (MA) experiments. Yet the fitness effect of single mutations is rarely measured in MA experiments. This raises several issues, notably for inferring epistasis for fitness. The acceleration of fitness decline in MA lines has been taken as evidence for synergistic epistasis, but establishin...

suns. The extension of the Andromeda nebula beyond the photographic limits is presumably typical of what may be found for other such objects. Comparisons with the surface brightness of the galaxy are still hampered by uncertainties in the data for our own system. There may be no part of the galaxy which, seen from outside, would be as bright as the central portion of M 31. In the latter system ...

H A P L O I D vertebrates may occur spontaneously but are very rare (Fankhauser, 1941; Humphrey & Fankhauser, 1957); however, haploids may be experimentally produced in fish (Swarup, 1959) and in mammals (Beatty, 1953), while amphibian eggs may be so treated that all developing embryos are haploid (Porter, 1939; Gurdon, 1960). The full descriptions of the development of haploid Rana pipiens (Po...

Doubled haploids (DH) have become a powerful tool to assist in different basic research studies, and also applied research. The principal (but not the only) routine use of DH by breeding companies is produce pure lines for hybrid seed production crop species. Several decades after discovery haploid inducer maize anther culture as method plants from pollen precursors, biotechnological revolution...

Chromosome doubling of microspore-derived plants is an important factor in the practical application of microspore culture technology because breeding programs require a large number of genetically stable, homozygous doubled haploid plants with a high level of fertility. In the present paper, 29 populations of microspore-derived plantlets from cabbage (Brassica oleracea var. capitata) and brocc...

We tested the hypothesis that the time course of the evolution of antifungal drug resistance depends on the ploidy of the fungus. The experiments were designed to measure the initial response to the selection imposed by the antifungal drug fluconazole up to and including the fixation of the first resistance mutation in populations of Saccharomyces cerevisiae. Under conditions of low drug concen...