A new arylnaphthalene lignan glycoside, patavine (1), together with five known lignans, justicidin B (2), diphyllin (3), tuberculatin (4), majidine (5), and arabelline (6) were isolated from shoot cultures of Haplophyllum patavinum. The structure of the new compound was elucidated by extensive one-dimensional (1D) and two-dimensional (2D) NMR experiments and mass spectrometry. The cytotoxicity ...

A major gene termed Hyp-1 encoding for hypericin (HyH) biosynthesis was cloned and characterized from Hypericum perforatum (St. John's wort) cell cultures. H. perforatum leaves are widely used as an herbal remedy in the treatment of mild to moderate depression. Hypericin, a photosensitive and red-colored naphthodianthrone, has been reported as the bioactive compound responsible for reversing th...

Background and objectives: Haplophyllum canaliculatum is an endemic and endangered Iranian plant from Rutaceae family. The object of this work was to study the volatile production in established shoot and callus cultures of Haplophyllum canaliculatum as well as isolation, identification and sequencing of 18S rRNA gene from callus culture. Methods: Shoot and callus cultures of H. canaliculatum w...

The active medicinal constituents in Hypericum perforatum, used to treat depression and skin irritation, include flavonoids and xanthones. The carbon skeletons of these compounds are formed by chalcone synthase (CHS) and benzophenone synthase (BPS), respectively. Polyclonal antisera were raised against the polyketide synthases from Hypericum androsaemum and their IgG fractions were isolated. Im...

The essential oils obtained by hydrodistillation from the aerial parts of Hypericum perforatum L. and Hypericum scabrum L. from plants growing wild in Tajikistan were analyzed by GC-MS. Sixty-six compounds were identified in the essential oils of H. perforatum with germacrene D (13.7%), α-pinene (5.1%), (E)-caryophyllene (4.7%), n-dodecanol (4.5%), caryophyllene oxide (4.2%), bicyclogermacrene ...

The dried aerial parts of Hypericum perforatum, gathered during the flowering period or shortly before, are used medicinally. The generic name of the herb derives from the Greek meaning to ‘overcome an apparition’ and in earlier times homes would have a plant hanging over the door to ward off evil spirits. This species of hypericum (H. perforatum) is referred to as perforate St John’s wort due ...

Investigations have been made to develop an efficient protocol for identification and quantification of phenolic acids and flavonoids in hairy roots (HR) of Hypericum perforatum L. HR were induced from root segments of in vitro grown seedlings from H. perforatum, after co-cultivation with Agrobacterium rhizogenes A4. Transgenic status of HR was confirmed by PCR analysis using rolB specific prim...

Background: Flavonoids of Hypericum perforatum are important secondary metabolites which have been widely utilized in medicine for a range of purposes. The use of methyl jasmonate (MeJA) elicitation for the enhancement of flavonoid production in cell suspension culture of H. perforatum would be an efficient alternative method for the flavonoid production. Results: MeJA influenced the cells grow...