Methods nCor a 8 was purified from raw hazelnuts by precipitation and chromatographical steps. Subsequently, the protein was identified by N-terminal sequencing and mass spectrometry. Stability of the protein was investigated by simulated gastric and duodenal digestion assays. Hazelnut lipids were extracted employing the hexane method. Finally, IgE binding activity of native and reduced/alkylat...

Methods Otherwise healthy children with oral food challenge confirmed hazelnut allergy were prospectively evaluated. Crude protein extracts were obtained from 5 hazelnut varieties, including autochthon, Northern Italy and Oregon (USA) cultivars, with phosphate saline buffer, pH 7.2. The immunoreactive protein components were identified by SDS-PAGE electrophoresis and Western immunoblotting, usi...

Methods Thirty-five children (median age: 8.3, range2.2–14.2 yr) reported with immediate reactions to hazelnut ingestion underwent food challenge (DBPCFC) between April 2007 and May 2012. Skin prick test (SPT), specific IgE determination at ImmunoCAP with whole food allergens and with allergen components at ImmunoCAP ISAC microarray chip were performed. The molecular profile of reactivity was c...

Extraction of high quality RNA is a critical step in molecular genetics studies. Hazelnut is one of the most important nuts plants in the world. The presence of the taxol and other taxanes in hazelnut plant necessitates explaining their biosynthesis pathway and identifying the candidate genes. Therefore, an easy and practical method is necessary for RNA extraction from hazelnuts. Hazelnut has h...

The Agricultural Reform Implementation Programme (ARIP), which was adopted under the aegis of a World Bank-financed Structural Adjustment Programme between 2001-2008, aimed to ‘lock in’ the neoliberal hegemony that had been developing in Turkey since the early 1980s by transforming the nation’s agrarian political economy. For hazelnut-producing petty peasant groups of the Black Sea Region, this...

The pyrolysis of hazelnut shell, polyethylene oxide and their blends were carried out catalytically at 500 and 650 oC. Potassium dichromate was chosen according to its oxidative characteristics and decomposition temperature (500 oC) where decomposition products are CrO3 and K2CrO4. As a main effect, a remarkable increase in gasification was observed using this catalyst for pure components and b...

Background Mast cells are activated during allergic reactions, releasing inflammatory mediators that include tryptase. This process leads to the symptoms of an allergic reaction. A transient increase in the total level of serum tryptase indicates mast cell activation, e.g. an anaphylactic reaction. This may help to confirm and assess the extent of a systemic reaction. In the current study, the ...

self pollinated cultivars of hazelnut are limited mainly due to dichogamy and self- incompability. to enhance productivity of hazelnut orchards, identification and selection of suitable polinizers are necessary. to meet this quest, collection and evaluation of iranian hazelnut germplasm have began by department of horticulture research, seed and plant improvement institute, iran, since 1994. ha...

In this work the Origanum vulgare leaf extract was used to green synthesis of Ag nanoparticles (NPs) supported on Hazelnut shell as an environmentally benign support. The Ag NPs/Hazelnut shell as an effective catalyst was prepared through reduction of Ag+ ions using Origanum vulgare leaf extract as the reducing and capping agent and Ag NPs immobilization on Hazelnut shell surface in ...

Performance of commercially available component resolved microarray has not been widely evaluated in food allergy. Nut allergy is a prevalent and increasing food allergy in which component resolved diagnosis is crucial. In the context of a multicentric study on pollen and vegetable food allergy in Spain, we enrolled patients (n=100) who had suffered at least two episodes of immediate symptoms a...