A diluted solution of a mixture of Hb S and Hb A in concentrated phosphate buffer was found to aggregate with a clear demonstration of delay time. The mechanism of the participation of Hb A in the aggregation of deoxy Hb S in concentrated phosphate buffer has been investigated. Both the delay time and the aggregation time in the polymerization reaction of mixtures of Hb A and Hb S were prolonge...

The functional properties of four glycosylated minor components of human hemolysate have been examined. In 0.1 M potassium phosphate buffer, pH 7 and 2O”C, the p50 values of Hb Al,,, Hb Alaz, Hb Al,, and Hb A, were 14,9.8,6.3, and 7.4 mm Hg, respectively, compared to 7.8 mm Hg for the major component, Hb Ao. The A log p&A pH (pH 7 to pH 8) was -0.5 for Hb A1 .1, Hb Alaz, and Hb A1, as compared ...

BACKGROUND Cation exchange-high performance liquid chromatography (CE-HPLC) is most commonly used to evaluate hemoglobin (Hb) variants, which elute in the Hb A2 window. This study aimed to assess prevalence of an uncommon Hb variant, Hb D-Iran, and compare its red cell parameters and peak characteristics with those of Hb E that commonly elutes in the Hb A2 window. METHODS Generally, we assess...

The authors describe a modification of the instrumental parameters of the Diamat fully automated HPLC system for Hb A(2) assay (Bio-Rad Laboratories, Milan, Italy) in order to obtain simultaneous determination of Hb A(2) and Hb F.Hb A(2) and Hb F measurements are reproducible (within-run CV 2.6%, with Hb A(2)2.7%; 5.1%, with Hb F 1.3%) and accurate (from a comparison with two microchromatograph...

The purpose of this research was to evaluate the combined effects of turkey hot-boning and cold-batter mincing technology on acceleration of meat turnover and meat quality improvement. For each of 3 replications, 15 turkeys were slaughtered and eviscerated. Three of the eviscerated carcasses were randomly assigned to water-immersion chilling for chill-boning (CB) and the remaining were immediat...

Intravenous administration of cell-free Hb induces vasoconstriction and circulatory disorders, presumably because of the intrinsic affinities to endogenous nitric oxide (NO) and carbon monoxide (CO) as vasorelaxation factors and because of the facilitated O(2) release that might induce autoregulatory vasoconstriction. We examined these gas reactions when Hb-containing solutions of four kinds we...

OBJECTIVE Advanced glycation end products (AGEs) are a risk factor for diabetic complications. We have developed an assay method for N-(carboxymethyl)valine (CMV) of the hemoglobin (CMV-Hb), which is an AGE generated from HbA1c. Herein we describe the clinical utility of CMV-Hb measurement for the diagnosis of diabetic nephropathy RESEARCH DESIGN AND METHODS BALB/c mice were immunized with ca...

Recent interest in therapies for sickle cell anemia based on elevating fetal Hb has made accurate estimates of the sparing effect of fetal Hb (Hb F) and other non-sickle Hbs on sickle Hb (Hb S) polymerization essential. We have developed a technique, using HbCO as surrogate for HbO2, that enables us to assess the solubility of Hb S as a function of ligand saturation under conditions that mimic ...