the major lipoprotein classes are heterogenous. human serum high-density-lipoproteins (hdl) are separated into two major subfractions (hdl2, d=1.063-1.125 g/ml, hdl3, d=1.125-1.210 g/ml) by a simple double precipitation method. there was a strong inverse relation between hdl-cholesterol (hdl-c) and subfractions hdl2-c and hdl3-c with atherosclerosis. this study demonstrated that hdl-c (p<0.001)...

Lipoproteins modulate innate and adaptive immune responses. In the chronic inflammatory disease multiple sclerosis (MS), reports on lipoprotein level alterations are inconsistent and it is unclear whether lipoprotein function is affected. Using nuclear magnetic resonance (NMR) spectroscopy, we analysed the lipoprotein profile of relapsing-remitting (RR) MS patients, progressive MS patients and ...

Cholesteryl ester transfer protein (CETP) deficiency causes elevated high-density lipoprotein-cholesterol (HDL-C) levels; its impact on HDL functionality however remains elusive. We compared functional and compositional properties of HDL derived from 9 Caucasian heterozygous CETP mutation carriers (splice-site mutation in intron 7 resulting in premature truncation) with those of 9 age- and sex-...

It is well-known that oxidative stress and inflammatory processes are linked to the incidence of atrial fibrillation (AF). In order to provide prognostic biomarkers for AF based on lipoprotein levels, we compared the lipid and protein parameters of oxidation and inflammation in individual lipoproteins from middle-aged females with AF. We analyzed plasma and lipoproteins (VLDL, LDL, HDL2, HDL3) ...

In this study we determined in vivo conversions of human ’H-labeled cholesteryl ester-labeled HDL3 ([’HICEHDLs) in male rats and the effects of partially purified lipid transfer protein on the conversion processes. Zonal centrifugation techniques were used to prepare the [’HICE-HDLS and to follow the conversion processes. One hour after the injection, a complete conversion of HDLs to and HDL,-d...

Cholesterol efflux from cultured cells can be mediated through binding of high density lipoprotein (HDL) to a cell-surface site which shows many characteristics of a biological receptor. To determine whether a specific protein forms a component of this site, cell membrane proteins were analyzed by ligand blotting using 125I-HDL3. Results demonstrated that membranes from a number of cell types p...

sumac (rhus coriaria l.) is used as an herbal remedy in traditional medicine. the aim of this study was to determine the effects of sumac (rhus coriaria l.) on serum glycemic status, apolipoprotein (apo) b, apoa-i and total antioxidant capacity (tac) in type 2 diabetic patients. this double blind randomized controlled clinical trial was conducted on 41 type 2 diabetic volunteers randomly assign...

This study was performed to determine relations among concentrations of high-density lipoprotein (HDL) apolipoprotein (apo) A-I and apoA-II and lipoproteins with apoA-I only (LpA-I) and with both apoA-I and apoA-II (LpA-I:A-II) in patients with low plasma levels of HDL cholesterol. Seventyseven middle-aged men with low HDL cholesterol levels (<40 mg/dL) were compared with 37 middle-aged men wit...

Interactions of high density lipoproteins (HDL) with very low (VLDL) and low (LDL) density lipoproteins were investigated during in vitro lipolysis in the presence of limited free fatty acid acceptor. Previous studies had shown that lipid products accumulating on lipoproteins under these conditions promote the formation of physical complexes between apolipoprotein B-containing particles (Biochi...

We have previously shown that hepatic lipase (HL) is inactive when bound to purified heparan sulfate proteoglycans and can be liberated by HDL and apolipoprotein A-I (apoA-I), but not by LDL or VLDL. In this study, we show that HDL is also able to displace HL directly from the surface of the hepatoma cell line, HepG2, and Chinese hamster ovary cells stably overexpressing human HL. ApoA-I is mor...