The objective of the present study was to examine the effects of chilling period on semen quality of Sannan and Jamunapari. Semen of goat breeds were collected with artificial vagina and chilled at 5-9˚C. Volume, colour and pH were determined on fresh semen. After dilution, microscopic examination was performed for the progressive motility and sperm viability. Diluted semen of both breed was co...

1. Washed erythrocytes, in both acid and alkaline solutions, hemolyze more rapidly when allowed to settle out on a clean microscope slide than when kept in suspension. 2. This is also true on slides coated with paraffin, paraffin oil, or vaseline, and on mica surfaces. 3. The presence of as little as 0.1 per cent serum inhibits such contact hemolysis, particularly in alkaline solutions. 4. Cont...

Dysuria is a common presenting complaint of women and urinalysis is a valuable tool in the initial evaluation of this presentation. Clinicians need to be aware that pyuria is the best determinate of bacteriuria requiring therapy and that values significant for infection differ depending on the method of analysis. A hemocytometer yields a value of > or = 10 WBC/mm3 significant for bacteriuria, w...

In a previous paper (1) a method has been described for measuring the adhesiveness of leucocytes to glass surfaces, and a series of results obtained by this method was reported. As a further calibration of the method it was necessary to determine what variations in adhesiveness could be produced by varying the H ion concentration. Since the method was originally devised as a means of comparing ...

A highly significant correlation was detected among photometric and direct sperm enumeration techniques for evaluating sperm concentration. Depending on the instrument used, the photometric techniques tended to overestimate or underestimate sperm concentration when compared to a hemacytometer standard. Precautions should be taken when using techniques other than a hemacytometer to measure sperm...

The Coulter Counter IIA, an automatic particle counter, was a fast, accurate device for counting spermatozoa in fire ants. Queens of Solenopsis invicta, the red imported fire ant (RIFA) had an average 5,669,683 ? 48,148 spermatozoa in the spermatheca two weeks after mating. RIFA males, ready for a nuptual flight, had an average of 8,761,637 spermatozoa. Males and females of S. richteri, the bla...

In studies of both the establishment and breakdown of cnidarian-dinoflagellate symbiosis, it is often necessary to determine the number of Symbiodinium cells relative to the quantity of host tissue. Ideally, the methods used should be rapid, precise, and accurate. In this study, we systematically evaluated methods for sample preparation and storage and the counting of algal cells using the hemo...