In an earlier report, it was shown that murine spleen cells cultured with concanavalin A (Con A) released into the culture supernatants helper and suppressor substances for antibody production. The present communication describes the production of rabbit antisera against culture supernates from Con A-activated spleen cells and their use in a plaque assay for mitogen-activated T cells. The plaqu...

aeromonas hydrophila secretes several extracellular proteins including enterotoxin, hemolysin and aerolysin that are associated with the bacterial virulence. previous studies have shown that two hemolytic toxins, hemolysin a and aerolysin a contribute to the virulence of aeromonas hydrophila. in the current study, a total of 50 strains of aeromonas hydrophila, including 28 (56%) strains isolate...

The hemolytic activity of 20 classical and 3 El Tor strains of V. cholerae O1 was examined phenotypically and genetically. The El Tor strains lysed bovine, chicken, human, rabbit, and sheep erythrocytes (RBCs), while the classical strains lysed only chicken and rabbit RBCs. The assay was done with RBCs in Tris-NaCl buffer, since phosphate-buffered saline was found to inhibit hemolytic activity....

A 15-month-old male baby of Chinese ethnicity was brought by his parents to the emergency department of our hospital with lethargy and yellowish discoloration of eyes, noticed for ;24 hours. On examination, the baby was pale and icteric. Blood tests revealed a low hemoglobin of 50 g/L, mild neutrophilia, and normal platelet count. A blood film examination showed normocytic normochromic red cell...

The European spider Tegenaria agrestis (Walckenaer) (hobo spider) has been implicated as a spider of medical importance in the Pacific Northwest since its introduction in the late 1980s. Studies have indicated that the hobo spider causes necrotic tissue lesions through hemolytic venom or through the transfer of pathogenic bacteria introduced by its bite. Bacterial infections are often diagnosed...

FUKUOKA, Y., OKUDA, T. and TACHIBANA, T. Variance in the Activity o f the Fourth Component of Mouse Complement. Tohoku J. exp. Med., 1983, 141 (2), 225-235 There are two methods for hemolytic titration of 04. One is a method using EACIgP, °XyC2hu and EDTA-CSP (E-C method) and the other using EACIgP, oxyC2hu and C4DSP (C4D mehtod). The 04 titers obtained by these two methods differed depending o...

The complement system plays an important role in host defense against infection and in most inflammatory processes. The standard 50% hemolytic complement (CH50) assay is the most commonly used method of screening patient sera for functional activity of the classical complement pathway. Our objective in this study was to compare two newer methods (the enzyme immunoassay and the liposome immunoas...

Serum haptoglobin has been advocated as an indicator of intravascular hemolysis. We have evaluated a nephelometric determination of serum haptoglobin. The assay is sensitive and exhibits within-run precision in the range of 2.5-7.4% coefficient of variation (CV) and between-run precision of 7.0% (CV). In addition, when haptoglobin values determined with the nephelometric assay were compared wit...

Skalka B.: Antagonistic Effect of Staphylococcus hyicus and Staphylococcus chromogenes Exhibited on Staphylococcal Delta Hemolysin. Acta vet. Bmo, 60,1991: 61-69. Staphylococcus hyicus and Staphylococcus chromo genes are able to inhibit hemolytic effect of the staphylococcal delta hemolysin on agar media supplemented with human or sheep erythrocytes. This inhibition takes place even when the ef...

Glucose-6-phosphate dehydrogenase (G6PD) deficiency, an X-linked disorder, is usually observed in hemizygote males and very rarely in females. The G6PD class 1 variants, very uncommon, are associated with chronic hemolytic anemia. Here we report a Portuguese woman who suffered in her sixties from a chronic hemolytic anemia due to G6PD deficiency. Molecular studies revealed heterozygosity for an...