A total of 16 targeted oleanolic acid–uracil/thymine hybrids were designed and synthesized as potential cytotoxic agents. Most IC50 values were under 10.0 μM, with some of them under 0.1 μM in vitro test against tested cells (Hep-G2, A549, BGC-823, MCF-7 and PC-3). These hybrids displayed much more potent inhibitory activities compared with oleanolic acid and commercial anticancer drug 5-fluoro...

Confluent monolayers of the human hepatoblastoma-derived cell line, Hep G2, were incubated in serum-free medium. Conditioned medium was ultracentrifugally separated into d < 1.063 g/ml and d 1.063-1.20 g/ml fractions since very little VLDL was observed. The d < 1.063 g/ml fraction was examined by electron microscopy; it contained particles of 24.5 * 2.3 nm diameter, similar in size to plasma LD...

Abstract Background Clerodendrum is a genus of about 500 species belongs to the family Lamiaceae. Many this have been proved for treatment various diseases. This study was aimed evaluate cytotoxic effect different solvents and their most active fractions thomsoniae Balf.f. in human cancer cell lines. Aerial parts plant were subjected Soxhlet extraction. Phytochemical analysis done by using stan...

Plasmid DNA/galactosylated poly-L-lysine(GalPLL) complex was used to transfer luciferase reporter gene in vitro into human hepatoma cells by a receptor-mediated endocytosis process. DNA was combined with galPLL via charge interaction (DNA:GalPLL:fusogenic peptide, 1:0.4:5, w/w/w) and the resulting complex was characterized by dynamic light scattering, gel retardation assay and zeta potential an...

In the framework of Regge phenomenology, masses of the charmed states cq̄ (q = u, d, s) lying on the 13S1-like trajectories are estimated. The overall agreement between our estimated masses and the recent predictions given by modified quark models[hep-ph/0605019, hep-ph/0608011, hep-ph/0608139] is good. Masses of the observed charmed statesDs0(2317), DsJ (2860) and DsJ(2690)/DsJ(2700) can be rea...

Receptor-mediated endocytosis of tissue-type plasminogen activator (t-PA) was characterized with the human hepatoma cell line Hep G2. At 4 degrees C binding of 125I-t-PA to Hep G2 cells is rapid, specific, saturable, and reflective of a homogeneous population of 76,000 high-affinity surface sites per cell (Kd = 3.7 nM). The kinetics of 125I-t-PA binding to its receptor are characterized by rate...

The equations of motion of the massless sector of the two dimensional string theory, obtained by compactifying the heterotic string theory on an eight dimensional torus, is known to have an affine o(8, 24) symmetry algebra generating an O(8, 24) loop group. In this paper we study how various known discrete Sand T duality symmetries of the theory are embedded in this loop group. This allows us t...

In the present study the effects of three flavonoids on the repair of H2O2-induced DNA strand breaks were investigated in Caco-2, Hep G2, and V79 cells. At the concentrations used, myricetin, quercetin, rutin, and H2O2 did not significantly affect cell viability in all the cell lines. Catalase activity was measured in V79 cells and was found to be considerably lower than activities previously m...