The present study evaluated thephenolics composition and biological activities of various extracts Cyclamen coum. antioxidant properties these plant wasdetermined using the scavengingmethods (DPPH andABTS), total test systems (β-carotene/linoleic acid), reducing power (FRAP) assays.The antibacterial effect was examined with MIC method. phenolics were identified HPLC brine shrimp lethality used ...

BACKGROUND In the European Union, various fluoroquinolones are authorised for the treatment of food producing animals. Each administration poses an increased risk of development and spread of antimicrobial resistance. The aim of this study was to investigate the impact of parenteral administration of enrofloxacin on the prevalence of enrofloxacin and ciprofloxacin susceptibilities in the commen...

Methanolic extracts of flowers and leaves of Gentiana lutea L., together with the isolated compounds mangiferin, isogentisin and gentiopicrin, were used to investigate the antimicrobial activity of the plant. A variety of Gram-positive and Gram-negative bacteria as well as the yeast Candida albicans has been included in this study. Both extracts and isolated compounds showed antimicrobial activ...

This study aimed to investigate the population pharmacokinetics of difloxacin in crucian carp (Carassius auratus) orally provided a single dose 20 mg/kg body weight (BW). To achieve this, fish were sampled at various intervals using sparse sampling strategy, and plasma samples analyzed high-performance liquid chromatography (HPLC) method. Subsequently, naïve average data non-compartmental metho...

Methods We reviewed data from critically ill cirrhotic patients and matched controls in whom routine therapeutic drug monitoring (TDM) of broad-spectrum b-lactam antibiotics (ceftazidime or cefepime, CEF; piperacillin/tazobactam; TZP; meropenem, MEM) was performed. Serum drug concentrations were measured twice during the elimination phase by high-performance liquid chromatography (HPLC-UV). Ant...

Two novel butyrolactone antibiotics, cedarmycins A and B, were isolated from the cultured broth of the actinomycete Streptomyces sp. TP-A0456. The new compounds were purified by HP-20 resin, silica gel, ODS column chromatographies and preparative HPLC, consecutively. The structure of cedarmycin was determined by spectroscopic methods as an alpha,beta-unsaturated butyrolactone with a fatty acid ...

Penetration of linezolid into osteo-articular tissue and fluid was studied in 10 patients undergoing primary total knee replacement. Linezolid 600 mg 12 hourly was given orally over the 48 h before operation and intravenously 1 h before induction of anaesthesia. Mean concentrations of linezolid at 90 min after the final dose, in serum, synovial fluid, synovium, muscle and cancellous bone, assay...

Previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from Brazilian forests' plants. In the present study, these extracts were subjected to microdilution broth assay (MDBA) and disk diffusion assay (DDA) using planktonic Enterococcus faecalis ATCC(®) 29212™ and were submitted to phytochemical analysis in TLC and HPLC. Three extracts obtain...

Commiphora leptophloeos is a plant specie usually known for its medicinal purposes in local communities in Northeast Brazil. In order to evaluate its therapeutic potential, we aimed to determine the phytochemical and antimicrobial properties of C. leptophloeos extracts. Thin Layer Chromatography (TLC) was able to detect the presence of phenolic compounds, flavonoids and reducing sugars. Three p...