The reaction of the hydroxyl radical, generated by a Fenton system, with pyrimidine deoxyribonucleotides was investigated by using the e.s.r. technique of spin trapping. The spin trap t-nitrosobutane was employed to trap secondary radicals formed by the reaction of the hydroxyl radical with these nucleotides. The results presented here show that hydroxyl-radical attack on thymidine, 2-deoxycyti...

The methanolic extract of Sida retusa Linn.(Malvaceae),Urena lobata Linn.(Malvaceae)and Triumfetta rhomboidea Jacq.(Teliaceae) roots were found to inhibit lipid peroxidation, scavenge hydroxyl and superoxide radicals in vitro. The quantity of S.retusa root extract required for 50% inhibition of lipid peroxidation, scavenging hydroxyl radical and superoxide radical was 1130.24 ug/ml respectively...

Present study was designed to compare antioxidant and DNA damage protection of Soxhlet assisted (80% methanol) extract (SEMC) with microwave assisted (80% methanol) extract of Michelia champaca Linn. (MEMC). The in vitro antioxidant activity of SEMC and MEMC were tested for DPPH, hydroxyl radical (OH) by deoxyribose, lipid peroxidation and reducing power capacity. The extracts were also tested ...

This study investigated the effect of recombinant human tumor necrosis factor (rh I NT') on hydroxyl radical production by established cell lines in vitro, and its implication in the killing of tumor cells by rhTNF. During incubation of TNF sensitive mouse tumorigenic fibroblast L-M cells (2 x 10"cells) in the presence of rhTNF (100 U), hydroxyl radical produc tion as detected by the evolution ...

A vast majority of the studies addressing the free radicals including hydroxyl radical is a damage compound of biochemical molecules such as DNA, proteins and lipids. When free radicals specially hydroxyl radical are not adequately removed from the body, it may damage biological macromolecules, leading to a variety of disease occurs. Therefore, the body should be protected by an enzymatic or no...

BACKGROUND Acid electrolyzed water (AEW), which is produced through the electrolysis of dilute sodium chloride (NaCl) or potassium chloride solution, is used as a disinfectant in various fields because of its potent antimicrobial activity. The hydroxyl radical, an oxygen radical species, is often suggested as a putative active ingredient for AEW antimicrobial activity. METHODOLOGY/PRINCIPAL F...

The antioxident effects of crude mucilages (CM) and partially purified mucilages (PPM) from three different Taiwanese yam cultivars—including Dioscorea alata L. cv. Tainong 1 (TN1), Dioscorea alata L. cv. Tainong 2 (TN2), and D. alata L. var. purpurea (Roxb.) Ming-Jen (MJ)—were evaluated, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and superoxide radical scavenging...

Bradykinin relaxes arterioles on the brain's surface. This response is endothelium-dependent. The data presented here confirm the hypothesis that hydroxyl free radical mediates this response and may be the endothelium-dependent relaxing factor for bradykinin in this microvascular bed. The response to a locally applied bolus of bradykinin (80 micrograms/ml) was monitored by intravital TV microsc...

The present study was carried out to evaluate the antioxidant and free radical scavenging activity of methanolic extract of Ervatamia coronaria leaves (Apocynaceae) in various systems. DPPH radical, superoxide anion radical, nitric oxide radical and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the extract. The antioxidant activity of methanolic ex...

The hydroxyl radical and its reactivity within ice environments are crucial to many important atmospheric reactions. The associated molecular mechanisms are largely unknown due to challenges posed by direct experimental measurements and computational studies of this transient species. Here we report insights into the local structure and behaviour of the hydroxyl radical in bulk ice through an e...