Two 36-amino acid-containing peptides (al-CBZ), isolated from rat skin and tendon collagen by cleavage of the al chain with CNBr, were subjected to enzymatic hydroxylation with collagen proline hydroxylase. The peptides dithered initially only in that the ratio of hydroxyproline to proline was considerably lower in the tendon peptide, although the imino acid content of the two peptides was the ...

The activity of asiaticoside, isolated from Centella asiatica, has been studied in normal as well as delayed-type wound healing. In guinea pig punch wounds topical applications of 0.2% solution of asiaticoside produced 56% increase in hydroxyproline, 57% increase in tensile strength, increased collagen content and better epithelisation. In streptozotocin diabetic rats, where healing is delayed,...

Objective-To determine the effect of ultrasound therapy on hydroxyproline (HP) content in superficial digital flexor tendon (SDFT) in horses.Design - Experimental in vivo study.Animals - Eight adult castrated horses.Procedure- The mediocaudal surface of right metatarsal bone in was exposed and after complete separation of SDFT, it was splitted (striking 15 times) in full thickness in longitudin...

This study was designed to assess the efficacy of vacuum sealing drainage (VSD) on skull exposure wounds in rabbits and to investigate the underlying mechanism of the process. Full-thickness excisional circular wounds 2×2 cm with or without periosteum involvement were created in 88 New Zealand white rabbits (mean body weight: 3.0±0.65 kg). Animals were randomly divided into 4 groups: periosteum...

Hamiltonia suaveolens, Sphaeranthus indicus and Ziziphus jujuba Mill are one of the most important traditional medicinal plants. The primary indigenous use of these plants appears to be of the leaves, flowers and root as a topical treatment for wound healing. The Methanol extract of leaves, flower and root of these plants were used to evaluate the wound-healing activity in rats, using excision ...

Cardiac hypertrophy was produced in rats by constricting the abdominal aorta subdiaphragmatically. The weight of the left ventricle was significantly elevated 2 days after aortic constriction and was 19% higher than in shamoperated animals at 11 days. The activity of protocollagen proline hydroxylase (PPH), an enzyme participating in collagen biosynthesis, and the hydroxyproline content of the ...

Mature male Sprague-Dawley rats were subjected to an isoproterenol-induced myocardial infarction. Animals were sacrificed on a daily basis in order to assess the temporal changes in prolyl hydroxylase activity and collagen metabolism during the acute stages of myocardial necrosis and repair. Total myocardial hydroxyproline, as an indexof collagen content, increased promptly and markedly, beginn...

Passive stiffness and hydroxyproline content of myocardium hypertrophied by pressure-loading were determined in kittens 2, 8-16, and 24-52 wk after pulmonary artery banding, which initially elevated right ventricular systolic pressure by 10-15 mm Hg. Right ventricular mass increased by approximately 75%, three-quarters of which occurred during the first 2 wk after banding. Passive stiffness was...

Injury to the capillary endothelium and to alveolar epithelial cells of the lung may result in damage to the underlying collagen of the extracellular matrix. To examine this possibility, whole body irradiation, bleomycin injections, and exposure to hyperoxia were used to induce various types of lung damage in mice. The morphology of the lung and the cellular and protein content of bronchoalveol...