Abstract Purpose Determination of the association between ica genes and phenotypic biofilm formation in staphylococcal isolates involved conjunctivitis, their antibiotic resistance as well detection selected virulence characteristics: adhesion to epithelial cells vitro cytotoxicity. Methods The study included 26 Staphylococcus aureus (SA) epidermidis (SE) isolates. presence icaAD operon was det...

Biofilm formation in Staphylococcus epidermidis is mediated by icaADBC-dependent and -independent pathways. Subinhibitory concentrations of erythromycin, azithromycin, and clarithromycin enhanced, in a dose-dependent manner, the level of biofilm formation by 20% (21/105 isolates) by macrolide-resistant ica-positive and -negative isolates tested in vitro. The presence of ica, however, apparently...

The pathogenesis of Staphylococcus epidermidis is correlated with biofilm formation. We investigated the effect of three common alcoholic skin disinfectants, ethanol, n-propanol and isopropanol, on the biofilm formation of 37 clinical, icaADBC-positive S. epidermidis isolates. In alcohol-supplemented media 18 strains displayed increased biofilm expression. Sixteen of 19 strains were generally i...

objective(s) methicillin-resistant staphylococcus aureus (mrsa) is an important cause of nosocomial and community infections. biofilm formation, mediated by a polysaccharide intercellular adhesin (pia) and encoded by the ica operon, is considered to be an important virulence factor in both s. epidermidis and s. aureus. however, the clinical impact of the ica locus and pia production is less wel...

Background & Aims: Type 1 fimbriae is the most common adhesion factor in urine tract infection. In this Study, presence of virulence genes in isolated strains of uropathogenic E.Coli, O serotyping and molecular detection of phase variation of type 1 fimbriae were assessed during solid surfaces exposure. Methods: Isolated E.coli from urine samples of patients were serotyped by using serologic me...

Knowing the nucleotide sequence of the cholera toxin operon, we designed oligonucleotide primers for its-PCR amplification from local clinical isolates of V. cholerae. The resulting amplification product was cloned in a common pUC18 vector. Subsequently, a part of this operon encoding the cholera toxin Bsubunit (CTB) was reamplified and cloned between the BamH1 and EcoR1 sites of the same ...

There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to examine whether the duplicated copies have the same or different functions and gene...