A nonrad~oac t~ve DNA probe assay was developed to detect and ~ d e n t ~ f y infect~ous hernatopoiet~c necrosls virus (IHNV) uslng a dot blot format The probe a synthet~c DNA oligonuc leot~de labeled enzymatlcally w ~ t h biotln hybnd~zed spec~f~cal ly w ~ t h nucleocaps~d mRNA extracted from Infected cells early In the vlrus repl~cation cycle A r a p ~ d g u a n ~ d l n ~ u m th~ocyanate base...

Three strains of infectious hematopoietic necrosis virus (IHNV) were compared for their pathogenicity to alevin rainbow trout Oncorhynchus n~ykiss by waterborne exposure of the trout to approximately equal concentrations of each virus strain The Idaho strain of IHNV (Type 2) was the most virulent and induced a 62 % mortality over a 10 d period at water temperatures of 10°C. In contrast, strains...

Monoclonal antibodies to the nucleoprotein and glycoprotein of infectious haematopoietic necrosis virus (IHNV) were prepared. Hybridomas were selected by ELISA according to the production of murine immunoglobulins. The specificity of the monoclonal antibodies was tested by Western blotting after SDS-PAGE. The monoclonal antibodies reacted only with a panel of nine IHNV isolates differing in ele...

Forty-two infectious hematopoietic necrosis virus (IHNV) isolates from Alaska were analyzed using the ribonuclease protection assay (RPA) and nucleotide sequencing. RPA analyses, utilizing 4 probes, N5, N3 (N gene), GF (G gene), and NV (NV gene), determined that the haplotypes of all 3 genes demonstrated a consistent spatial pattern. Virus isolates belonging to the most common haplotype groups ...

Infectious hematopoietic necrosis virus (IHNV, Rhabdoviridae), is the causative agent of infectious hematopoietic necrosis (IHN), a disease notifiable to the World Organisation for Animal Health, and various countries and trading areas (including the European Union). IHNV is an economically important pathogen causing clinical disease and mortalities in a wide variety of salmonid species, includ...

Infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicaemia virus (VHSV) are rhabdoviruses that infect salmonids, producing serious economic losses. Two recombinant IHN viruses were generated by reverse genetics. For one (rIHNV GFP) the IHNV NV gene was replaced with the green fluorescent protein (GFP) gene. In the other (rIHNV-Gvhsv GFP) the G gene was also exchanged for t...

Neutralizing monoclonal antibodies were developed against strains of infectious hematopoietic necrosis virus (IHNV) from steelhead trout Salmo gajrdneri in the Deschutes River of Oregon, chinook salmon Oncorhynchus tshawytscha in the Sacramento h v e r of California, and rainbow trout Salmo gairdneri reared in the Hagerman Valley of Idaho. USA. These antibodies were tested for neutralization of...

The recently reported SAF-1 cell line from fins of gilt-head seabream was evaluated for susceptibility to lymphocystis disease virus (LDV) and to several salmonid fish viruses, such as infectious haematopoietic necrosis virus (IHNV), viral haemorrhagic septicemia virus (VHSV) and several strains of infectious pancreatic necrosis virus (IPNV). LDV, VHSV and IHNV replicated well in the cultured f...

A cell culture virus isolation procedure for infectious haematopoietic necrosis virus (IHNV) in the epithelioma papulosum cyprini cell line (EPC) is described. Ovarian fluid samples were collected from fish and tested for IHNV at 9 farms. The samples were inoculated in parallel on 24 h old EPC cell monolayers and in freshly trypsinized cells. The titre of the initial virus isolation and of firs...

The genome of infectious hematopoietic necrosis virus (IHNV), a salmonid novirhabdovirus, has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo By reverse genetics, eight recombinant IHNVs (rIHNVs), termed NxGy according to the respective positions of the nucleoprotein (N) and glycoprotein (G) genes along the genome, ...