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A number of commercial software packages are currently available to perform digital two-dimensional electrophoresis (2D-GE) gel analysis. However, both the high cost of the commercial packages and the unavailability of a standard data analysis workflow, have prompted several groups to develop freeware systems to perform certain steps of gel analysis. Unfortunately, to the best of our knowledge ...
Scattering experiments in the transmission electron microscope (TEM) result in a large number of phase-identification challenges. This note is first in a series on the inverse-problems associated with Bayesian model-selection and parameter-estimation [1] from TEM data. In particular here we present a fast method for determining two g-vectors and an inter-spot angle from your diffraction and HRE...
Ca sparks are elementary Ca-release events from intracellular Ca stores that are observed in virtually all types of muscle. Typically, Ca sparks are measured in the line-scan mode with confocal laser-scanning microscopes, yielding two-dimensional images (distance vs. time). The manual analysis of these images is time consuming and prone to errors as well as investigator bias. Therefore, we deve...
DETECHIP ® is a molecular sensing array used for identification of a large variety of substances. Previous methodology for the analysis of DETECHIP ® used human vision to distinguish color changes induced by the presence of the analyte of interest. This paper describes several analysis techniques using digital images of DETECHIP ® . Both a digital camera and flatbed desktop photo scanner were u...
BACKGROUND Air pollution is associated with a high burden or morbidity and mortality, but exposure cannot be quantified rapidly or cheaply. The particulate burden of macrophages from induced sputum may provide a biomarker. We compare the feasibility of two methods for digital quantification of airway macrophage particulate load. METHODS Induced sputum samples were processed and analysed using...
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