Cells of Lactobacillus bulgaricus, Escherichia coli, and Kluyveromyces (Saccharomyces) lactis immobilized in polyacrylamide gel beads retained 27 to 61% of the beta-galactosidase activity of intact cells. Optimum temperature and pH and thermostability of these microbial beta-galactosidases were negligibly affected by the immobilization. Km values of beta-galactosidase in immobilized cells of L....

The use of algae to treat wastewater has been under investigation for decades. Laboratory experiments were performed to study the growth rate and nitrate and phosphate uptake of free and immobilized cells of saline tolerant, nitrogen fixing cyanobacterium Gloeocapsa gelatinosa. The immobilization was carried out in calcium alginate matrix. The immobilized cells were found to be highly effective...

Tauhe present work shows the relation between kefir fermentation ability and carbohydrate uptake rate. This was examined in a model system containing kefir co-culture and lactose in order to study fermentation induced by yeasts and bacteria at the same time. Lactose uptake rate was recorded by using (14)C-labelled lactose. The effect of lactose, cell concentration and pH on lactose fermentation...

Pesticides are normally used to control specific pests and to increase the productivity in crops; as a result, soils are contaminated with mixtures of pesticides. In this work, the ability of Streptomyces strains (either as pure or mixed cultures) to remove pentachlorophenol and chlorpyrifos was studied. The antagonism among the strains and their tolerance to the toxic mixture was evaluated. Re...

Immobilized cells are widely used in bioconversions to produce biological products as well as in wastewater treatment such as solvent removal from wastewater streams. In this work, a rate model is proposed to simulate this kind of process in an axial-flow fixed-bed column packed with porous particles containing immobilized cells. The transient model considered various mass transfer mechanisms i...

Microorganisms capable of degrading di-n-butyl phthalate (DBP) were immobilized by polyvinyl alcohol (PVA) and used for DBP degradation. The characteristics of DBP degradation by immobilized and free cells were investigated. The experimental results showed that the degradation rate o f immobilized cells was higher than that of free cells. The optimal pH and temperature for DBP degradation were ...

The present study focus on the biodegradation of trinitrotoluene (TNT) by Bacillus sp. YRE1 isolated from red effluent in free state and also cells immobilized on charcoal and polystyrene. TNT degradation was monitored weekly for 168 hours, at 262 nm. Immobilized Bacillus sp. YRE1 was checked for its ability to degrade TNT by exposing it to different temperatures. It was found that both charcoa...

Immobilized whole cells of Rhodotorula rubra MIM147 were used in a packed bed flow reactor for the enantioselective reduction ?-ketonitriles and obtainment key building block synthesis antidepressant drug duloxetine.

Cell aggregates have been applied in various fields such as regenerative medicine and drug toxicity testing. We shown that H-(Lys-Pro)12-OH (KP24), a repeating sequence of lysine (Lys) proline (Pro), induces uniformly sized cell simply by its presence suspension. In this study, we considered peptide could be to three-dimensional culture substrate can induce uniform immobilizing it on the substr...

In recent years immobilized cells have commonly been used for various biotechnological applications, e.g., antibiotic production, soil bioremediation, biodegradation and biotransformation of xenobiotics in wastewater treatment plants. Although the literature data on the physiological changes and behaviour of cells in the immobilized state remain fragmentary, it is well documented that in natura...