نتایج جستجو برای: immuno blot electrophoresis
تعداد نتایج: 102732 فیلتر نتایج به سال:
Counter immuno electrophoresis was done on concentrated urine samples of 166 children with lower respiratory tract infection for the detection of Streptococcus pneumoniae and haemophilus influenzae type b antigens. About 101⁄4 cases showed the presence of Strept. Pneumoniae Polysaccharide antigen and1.8% H. influenzae antigen. No growth was seen on blood culture in 91.8% while 0.8% showed Strep...
An antigen detection system, termed immuno-polymerase chain reaction (immuno-PCR), was developed in which a specific DNA molecule is used as the marker. A streptavidin-protein A chimera that possesses tight and specific binding affinity both for biotin and immunoglobulin G was used to attach a biotinylated DNA specifically to antigen-monoclonal antibody complexes that had been immobilized on mi...
We report the first strain of glycopeptide-resistant Enterococcus faecium from Europe that contains a vanD allele isolated from blood cultures of an immunocompromised patient hospitalized in a French university hospital. Based on phenotypic results, PCR sequencing, pulsed-field gel electrophoresis, and Southern blotting, the isolate was assigned to E. faecium with a chromosomally located VanD a...
Following an outbreak of fascioliasis in Anzali Harbor in 1988, 452 blood samples were collected using a multistage sampling method for hematological and serological studies. After determination of eosinophilia in the samples, antifasciolia antibodies in the sera were also determined using enzyme linked immuno sorbant assay (ELISA) and counter-current immuno electrophoresis (CCIE). 64.2% ...
This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists of resolving RNAs by gel electrophoresis, followed by transferring and fixing to nylon membranes as ...
Background: Cryptosporidiosis is prevalent in animals and human beings, both immuno-competent and immuno-compromised persons, worldwide. Epidemiologically, it is important to differentiate between human and animal species. The aim of this study is to define the utility of a 1055 bp fragment of 18s rRNA for differentiation of Human and Cattle Cryptosporidiosis. Material and methods: 1020 human...
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