Antibodies against phycobilisom es o f Porphyridium cruentum immunoreact with B-phycoerythrin from the same organism. A strong antigen-antibody com plex is not only formed with the native B-phycoerythrin but also when the chromophore is chemically reduced, split or even degraded with chromic acid. Characteristics o f precipitations and dissolutions in the presence of the chaotropic reagents ure...

Insoluble antigen and antibody derivatives were obtained when ethyl chloroformate was added to solutions of antigen or antibody. Evidence has been obtained to show that polymerization has occurred and that the protein molecules are linked covalently to one another. The immunoadsorbent properties of these insolubilized proteins were investigated. They were found to be efficient, specific, and st...

Nicotinic acetylcholine receptors (AChR) extracted from skeletal muscle of amputated human limbs in 2% Triton X-100 were purified by two different methods of affinity chromatography, agarose conjugated with monoclonal anti-AChR antibody or a-neurotoxin. After elution, with 1-2 M NaCl buffers from the immunoadsorbent and with carbamylcholine from the a-toxin gel, the preparations were purifi...