Purpose: To assess the role of Kruppel-like factor 7 (KLF7) in hemangioma (HA) progression, KLF7 expression and regulation downstream targets HA tissues hemangioma-derived endothelial cells (HemECs) have been evaluated.
 Methods: Quantitative polymerase chain reaction (qPCR) immunoblotting were performed to normal tissues. Endothelial isolated from tissues, Cell Counting Kit-8 assay...

Immunoblotting has provided a powerful and effective approach to dissection of the immune response to mycobacterial antigens in a situation in which the availability of isolated antigenic components is severely limited. The basic approach of blotting on to a solid-phase support has been used in combination with SDS/polyacrylamide-gel electrophoresis, t.l.c., recombinant DNA technology and T-cel...

Reagent production: selecting the strain, preparing the antigen and controlling quality The choice of the strain to use is an extremely critical point. The use of the B103 strain, the reference strain used by Regalla et al. in 1999 to develop the IBT method, is highly recommended and should be used to ensure consistent results among laboratories. The antigen is then prepared from a culture grow...

Linear IgA bullous dermatosis (LABD) is characterized by linear binding of at the epidermal basement membrane zone. The major autoantigens for LABD are 120 kDa LAD-1 and 97 LABD97, which shedding products BP180. In general, autoantibodies to them detected immunoblotting using cell extract or culture medium keratinocyte lines, such as HaCaT cells, but they not highly sensitive. Therefore, we sou...