Methods for detecting microbial antigens in clinical specimens offer an alternative to culture in the diagnosis of some sexually transmitted diseases. Developers of the immunologic methods are faced with a number of problems in evaluating the new tests. Traditionally, these tests are compared to culture as the "gold standard." Unfortunately, culture for Neisseria gonorrhoeae or Chlamydia tracho...

In the present study, the serum and cerebrospinal fluid of horses diagnosed with Setaria digitata cerebrospinal filariasis were analyzed by western blot. The results revealed S. digitata protein bands measuring 65, 34, 22, and 18 kDa in molecular weight. In particular, the 18 kDa band is a possible candidate for clinical immunodiagnosis on the basis of western blot findings.

1 Cancer Autoimmunity Research Laboratory, Department of Biological Sciences, University of Texas, El Paso, TX 79968, USA 2Department of Medical Oncology, The First Affiliated Hospital of Xi’an Jiao Tong University Medical Center, Shaanxi 710061, China 3Division of Hematology/Oncology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA 4Biotherapy Center and Department ...

Cysticercosis is an important parasitic zoonosis, caused by the infection of Cysticercus cellulosae, the larval stage of Taenia solium. This disease is a serious public health problem in pigs and human beings in many developing areas worldwide. Practical, rapid, and efficient pre-mortem diagnostic methods therefore are of great significance. The cysticercus antigens and immunodiagnosis methods ...

indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of h. contortus. plate elisa was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. indirect plate elisa was able to demonstrate...

Regarding harmful agents in livestock, parasites play an important role moreover it also effect 1/4th of the human populace by various zoonotic diseases (e.g. Toxplasma, Cryptosporidium, Trypanosomiasis, etc.). In livestock the parasites successful invade host immune responses, so early diagnosis of parasitic agents by various immunodiagnostic techniques is very important. Numerous immunologica...

The macrophage electrophoretic mobility (MEM) test of Field and Caspary did not clearly separate patients with ocular neoplastic disease from those with inflammatory disease, although there was some indication of discrimination between choroidal melanoma and ocular inflammation. In our hands the test failed to give a reproducible result for the immunodiagnosis of ocular malignancy. The techniqu...