The preparation of the first monoclonal antibody (MAb) against the orthomyxovirus-like infectious salmon anaemia (ISA) virus is described. Characterization of the MAb included isotyping, enzyme-linked immunosorbent assay (ELISA), immunofluorescent staining of virus infected cell cultures (SHK-1 cells), immunoelectron microscopy (IEM) of negatively stained virus preparations, virus neutralizatio...

An indirect immunofluorescent (IF) test with antigen of partially cleaned Pneumocystis carinii cysts was evaluated for sensitivity and specificity. The test was reactive at the 1:8 and above level for 44% of the persons who had suspected and confirmed infections, for 7.3% of the healthy contacts, and for 1.4% of the general population. Results of tests with sera from people with measurable anti...

A new immunofluorescent method (FIAX) was compared with a radioimmunoassay procedure for the determination of tobramycin serum concentrations. When assaying three tobramycin control sera repeatedly, within-run and run-to-run variations by the FIAX method were all within acceptable limits, and no statistically significant differences were found. Results of 155 sera from patients who had been tre...

Scrub typhus is common among patients with acute fever in rural areas of Thailand. The authors prospectively recruited patients with acute fever from provincial Thai army hospitals. Dot-ELISA test for scrub typhus was done in hospitals and then compared with standard immunofluorescent assay for diagnosis of scrub typhus. Among 178 patients, scrub typhus was diagnosed by immunofluorescent assay ...

In order to study and evaluate the indirect immunofluorescent test and the ratio of CD41ymphocytes to CD8 lymphocytes, 97 cases of positive candida cultures were selected from among 400 patients referring to the hospital and suspected of candidiasis. In this study 39% of the patients had an antibody titer less than 1: 160 No significant correlation was seen between canclidiasis and the resu...