Allotype suppression is a unique example in higher organisms of the regulation of gene expression by antibodies directed against the product of those genes. Both in rabbits and mice, prenatal and/or early postnatal exposure to anti-immunoglobulin allotype antibody suppresses production of the allotype in animals genetically capable of producing it. However, the long-lived or "chronic" suppressi...

immunoglobulin class switch recombination deficiencies (ig csr deficiencies) or hyper igm syndromes (higm) are a group of primary immunodeficiency diseases, characterized by defective cd40 signaling of b cells resulting into a csr and a somatic hypermutation. the affected patients are characterized with reduced serum levels of igg and iga, and normal or elevated level of igm, which lead to incr...

Sialyltransferase structures fall into either GT-A or GT-B glycosyltransferase fold. Some sialyltransferases from the Photobacterium genus have been shown to contain an additional N-terminal immunoglobulin (Ig)-like domain. Photobacterium damselae α2-6-sialyltransferase has been used efficiently in enzymatic and chemoenzymatic synthesis of α2-6-linked sialosides. Here we report three crystal st...

BACKGROUND During the development of a therapeutic antibody, large numbers of monoclonal antibodies are required to screen for those that are best suited for the desired activity. Although the single cell-based immunoglobulin variable gene cloning technique is a powerful tool, the current methods remain an obstacle to the rapid production of large numbers of recombinant antibodies. RESULTS We...

The primary antibody deficiency syndromes are characterised by recurrent respiratory tract infections and the inability to produce effective immunoglobulin (Ig) responses. The best-known primary antibody deficiencies are common variable immunodeficiency (CVID), X-linked agammaglobulinaemia (XLA), immunoglobulin G (IgG) subclass deficiency, and selective antibody deficiency with normal immunoglo...

The origin of immunoglobulin on the surface of TDL in the rat has been studied by comparing the binding of purified alloantibodies recognizing the Ig-1a allotype of rat light chain, with that of rabbit antirat Fab antibodies. Both reagents labeled all TDL from rats of the DA strain (Ig-1a) with two categories of cells being detected; one binding 100-2,000 molecules of antibody, the other 10,000...

The heavy light chain (HLC) immunoassay quantifies light chain types of each immunoglobulin (Ig), enabling the calculation of ratios of HLC pairs (Katzmann & Rajkumar, 2013). This assay measures the light chain types of each Ig (G/A/K), generating ratios of monoclonal Ig/background polyclonal Ig concentrations as paired ratios. It has shown clinical utility in multiple myeloma (MM), particularl...

Immunofixation (IFE) is a standard method for detecting monoclonal immunoglobulins and characterizing its isotype. Recently clonality can also be determined by using immunoglobulin (Ig) heavy chain/light chain immunoassays - HLC, HevyliteTM. HLC separately measures in pairs light chain types of each intact Ig class generating ratios of monoclonal Ig/uninvolved polyclonal Ig concentrations. Stud...

Conventional light and electron microscopic methods do not allow a differentiation of Tand B-cells. A clasdficntion of neoplastic lymphatic cells into one of these subpopulations is therefore impossible with common morphological methods. It was not until specific cell funktions, i e. immunoglobulin(IG)-production and the formation of surface-Ig(S-Ig)-receptors, were demonstrable that one could ...

In the mouse, antibody directed against an immunoglobulin allotype, Ig-1b, passed from mother to offspring or injected into neonates, suppresses synthesis of immunoglobulin carrying Ig-1b. In allotype homozygotes as well as heterozygotes the allotype suppression is manifested both by a delay of several weeks in attaining initial detectable allotype levels and a reduction in allotype level conti...