background: the homologous recombination (hr) is one of the conventional cloning methods for the production of recombinant dna. it is a quick method for in vivo dna cloning without using the high cost restriction enzymes. a few modifications in the cloning procedure can increase the efficiency of this method. m aterials and methods: in this study, effect of heating on the rate of the igg1 heavy...

We report on the in silico-initiated cloning and molecular characterization of CTXN3 (cortexin 3), a new human gene that was specifically expressed in the kidney and brain due to tissue-specific alternative exon 1 usage, on chromosome 5q23.2 using digital gene expression displayer (DGED) and a novel in silico cloning approach based on both expressed sequence tags (ESTs) and genomic sequence. Th...

Multiple Pregnancy-Associated Glycoprotein (PAG) genes, coding various chorionic products that belong to the aspartic proteinase family (AP), have been discovered in various eutherians (Placentalia). This paper presents cloning of five novel porcine PAG (pPAG) cDNAs: pPAG3, pPAG4, pPAG5, pPAG6, pPAG8 and pPAG10. All cDNAs have been identified by trophoblastic library screening on 13-17 days pos...

MOTIVATION Statistical analysis of links on the Semantic Web is important for various evaluation purposes such as quantifying an individual's scientific research output based on citation links. SPARQL has been proposed as a standardized query language for the Semantic Web and is intuitively understandable; however, it does not adequately support statistical evaluation of semantic links. RESUL...

Background: The modeling of Biological Regulatory Networks (BRNs) relies on background knowledge, deriving either from literature and/or the analysis of biological observations. However, with the development of high-throughput data, there is a growing need for methods that automatically generate admissible models. Methods: Our research aim is to provide a logical approach to infer BRNs based on...

INTRODUCTION Plasmepsin V (PM-V) have functionally conserved orthologues across the Plasmodium genus who's binding and antigenic processing at the PEXEL motifs for export about 200-300 essential proteins is important for the virulence and viability of the causative Plasmodium species. This study was undertaken to determine P. vivax plasmepsin V Ind (PvPM-V-Ind) PEXEL motif export pathway for pa...

Rice (Oryza sativa) is a staple food consumed by the majority of world’s population. Climate change, however, has created significant threat to our security as it posed severe effects on rice production. The emergence genome editing technology opened new era in crop improvement. Hence, this study aims develop CRISPR/Cas9 construct drought tolerance for O. sativa subsp. indica cv. IR64 using Gol...

Synthetic biology is an emerging field that combines molecular biology with engineering principles, which requires abstraction levels applied to a modular biological componentry. The Registry of Standard Biological Parts harbours such a repository of standardized parts, and thereby facilitates the combination of complex molecular modules to novel genetic circuits and devices. However, since fin...

background: bacterial superantigen staphylococcal enterotoxins (ses), has stimulated polyclonal t cells irrespective of their antigen specificity, resulted a massive release of cytokines, and suggested that they could be assigned as a candidate of new antitumor agents. recent attempts have done to specifically target superantigens towards tumors, subsequently monoclonal antibodies and tumor-rel...