Recent whole-genome analysis suggests that lateral gene transfer by bacteriophages has contributed significantly to the genetic diversity of bacteria. To accurately determine the frequency of phage-mediated gene transfer, we employed cycling primed in situ amplification-fluorescent in situ hybridization (CPRINS-FISH) and investigated the movement of the ampicillin resistance gene among Escheric...

The number of Enterobacteriaceae, with particular attention given to the presence of Escherichia coli and Klebsiella pneumoniae, was determined in hospital effluents and municipal wastewater after various stages of purification. The emission of these microorganisms to the ambient air near wastewater treatment plant (WWTP) facilities and to the river water, which is a receiver of the WWTP efflue...

BACKGROUND The aim of this study was to explore the plasticity and transdifferentiation potential of murine spermatogonial stem cells (SSCs) into hematopoietic cells. METHODS GFP(+)CD49f(+)H-2K(b-) SSCs of male donor mice were isolated and injected into the bone marrow (BM) of Busulfan-treated GFP(-) female mice. Twelve weeks post-transplantation, the recipients were sacrificed and their BM, ...

With the development of chemical synthetic strategies and available building blocks, in situ‐forming hydrogels have attracted significant attention in the biomedical fields over the past decade. Due to their distinct properties of easy management and minimal invasiveness via simple aqueous injections at target sites, in situ‐forming hydrogels have found a broad spectrum of biomedical applicatio...

sound power is considered as an excellent measure for evaluating the efficiency of noise controls as well as for comparing noise sources in workrooms. this parameter can be determined by sound pressure or intensity based methods in real conditions of workrooms. this paper aims empirically to compare these in situ methods in relation to a typical industrial machine located in workroom in terms o...

in this work, a thermoplastic polyurethane elastomer (tpue) model based on polytetramethylene glycol, toluene diisocyanate and 1,4-butanediol was selected and synthesized. according to this model two types of polyurethane nanocomposites were prepared by in situ polymerization and melt intercalation procedures. the organo-modified nanoclay was used in nanocomposites samples in 0.4 weight percent...

bis(2-r-ind)zrcl2 (r: h or phenyl) was supported on different types of silica by in situ impregnation method and used for ethylene polymerization. in this method, the step of catalyst loading on support was eliminated and common alkyl aluminum (triisobutylaluminum, tiba) cocatalyst was used instead of expensive methyl aluminiumoxane (mao) cocatalyst in the polymerization. the effect of surface ...