We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurot...

Despite their sustainability, the potential of biocatalytic processes in industrial production is far from being realized. The main challenges this field are development highly active, robust, and stable biocatalysts, efficient regeneration cofactors, prevention biocatalyst deactivation under harsh conditions. In addition to engineering, enzyme cell immobilization plays a crucial role process f...

This paper reviews general approaches for applying activated carbon (AC) amendments as an in situ sediment treatment remedy. In situ sediment treatment involves targeted placement of amendments using installation options that fall into two general approaches: 1) directly applying a thin layer of amendments (which potentially incorporates weighting or binding materials) to surface sediment, with...

Remediation of lead (Pb)-contaminated sites with phosphate amendments is one of the best studied and cost-effective methods for in situ immobilization. In this treatment, a very stable mineral, pyromorphite Pb5(PO4)3Cl, is formed. Several studies propose to improve this treatment method with the addition of phosphate-solubilizing bacteria (PSB). The effect of bacteria on solubilization of pyrom...

background: flat urothelial lesions comprise a spectrum of morphologic changes ranging from reactive atypia to carcinoma in situ (cis). urothelial dysplasia and cis are associated with the recurrence and progression of urothelial carcinoma. distinguishing cis and dysplasia from reactive atypia based on histolopathogical features alone is often difficult. using different immunohistochemical mark...

background: various frequencies of sperm aneuploidy are reported in sperms of subfertile patients compared to normal individuals. moreover, sperm dna damage is shown to be associated with male infertility. in this study, the rate of dna damage and frequencies of aneuploidy in sperms of subfertile patients was investigated. methods: semen samples were obtained from healthy normal and subfertile ...

two facile and low cost routes were used for the synthesis of if-ws2 nanoparticles. in this paper, preparation of if-ws2 nanoparticles with two different methods was investigated. in the first method, wo3 nanoparticles were reacted with h2s gas in n2/h2 reducing atmosphere at 900 oc. the as-synthesized if-ws2 nanoparticles are spherical shape with an average size of about 70 nm.  in the second ...

in this research, using pyrolysis of phenolic resin in the presence of silicon particles, the sic ceramic composite is formed. the samples were prepared by introducing 30, 35, 40, 45 and 50 wt% of si particles to the phenolic resin. the samples were cured at 180°c then carbonized at 1100°c. the final carbonized c/si composites are hot-pressed at 1500°c in inert atmosphere, which is more than th...

a new method was used to prepare abs/pa6 blends by in situ polymerization and compatibilization method. in this process, ε-caprolactam monomer was polymerized in presence of abs via activated anionic polymerization to produce pa6. the main ingredients of the reaction mixture included: monomer ()ε-caprolactam), catalyst na-caprolactamate), microactivator hdi)( and macro-activator sma)(. then, in...