The advancements made in tissue culture techniques has made it possible to regenerate various horticultural species in vitro as micropropagation protocols for commercial scale multiplication are available for a wide range of crops. Clonal propagation and preservation of elite genotypes, selected for their superior characteristics, require high degree of genetic uniformity amongst the regenerate...

Micropropagation of banana (Musa paradisiaca Linn.) var Grand naine was carried out with media sterilized by microwave radiation. Microwave oven was effectively used to sterilize the media for in vitro shoot-multiplication of banana against autoclave. Sterilization of media in microwave oven was successfully achieved at 900W/200 ml media (4 min). In vitro shoot-multiplication was higher in the ...

Background & Aim: Clematis is an ornamental and medicinal plant that is primarily planted as an ornamental plant. Clematis orientalis is native to Asia and Central Europe, used as an antiseptic and refrigerant. The propagation of Clematis is very important. In this study, the effects of plant growth regulators on rooting and growth of the plants in vitro cult...

Plant tissue culture is an alternative method of commercial propagation and is being used widely for the commercial propagation of a large number of plant species, including many medicinal plants. Somatic embryogenesis is a process by which asexual or somatic cells are induced to form embryos in culture. Somatic embryogenesis is a multi-step regeneration process starting with formation of pro-e...

In vitro micropropagation of wild cherry is presently one of the main commercial ways to clonally propagate this species (Cornu and Boulay, 1986). In order to extend this technique to a large number of clones, it seems necessary to improve our knowledge of the behavior of the explants during the in vitro culture. Since plant growth regulators (PGR) play an important role in this technique (Marg...

A substantial number of micropropagated plants do not survive transfer from in vitro conditions to greenhouse or field environment. The greenhouse and field have substantially lower relative humidity, higher light level and septic environment that are stressful to micropropagated plants compared to in vitro conditions. The benefit of any micropropagation system can, however, only be fully reali...