identification of exogenous factors affecting spermatogonial stem cells (sscs) proliferation in vitro, provides worthy ways to study the basic biology of the cells. the aim of this study was to investigate the effects of a gnrh analogue (alareline acetate) on sscs colonization in short-term co-culture with sertoli cells. five, three-month old holstein male calves were used to isolate spermatogo...

The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. To investigate and biomanipulation of these cells, proliferation and viability rate of cells should be ...

in vitro methods provide a variety of tools to supplement traditional methods for collection, propagation and preservation of endangered plant species. in this study, an efficient protocol was developed for in vitro propagation of colutea gifana, a rare and endangered plant species with limited reproductive capacity that grows in a narrow area of iran. single node explants were used for a serie...

objective: osteon has been introduced as a bone substitute material. it is biphasic calcium phosphate (bcp) that use in dentistry. the aim of the present study was to evaluate the effect of osteon on the proliferation, cell viability and differentiation of saos-2 cells in vitro. also it was compared with cerasorb bone graft. materials and methods: two different bone grafts materials, osteon an...

an efficient and cost effective protocol for in vitro propagation of pineapple (ananas comosus var. queen) has been developed. in the proliferation stage, agar based murashige and skoog (ms) media was supplemented with 3.0 mg/l benzyleaminopurine (bap), 0.5 mg/l indole acetic acid (iaa) and 50 mg/l adenine sulphate as rbc design experiment. two approaches were taken to reduce the chemical cost ...

in vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. micropropagation using nodal segments of rosa canina under different combinations of bap, ga3 and naa on ms and vs, medium was investigated. the results showed that the highest shoot proliferation was obtained on vs medium cont...

Traumatic brain injury (TBI) is one of the main causes of mortality and morbidity worldwide. Despite extensive investigations over the past few decades, no effective therapies exist to improve the brain function in patients with TBI. Neural tissue engineering is an attractive therapeutic approach to restore the brain structure and function of damaged tissue. Bioactive motif of Stromal cell-deri...

background to current knowledge, different oocyte's recovery method and various seasons have profound impact on in vitro embryo production (ivep). objectives the aim of this study was to define an efficient recovery method for oocytes harvesting from slaughterhouse material in different seasons, and their effects on ivep yield. materials and methods ovaries from slaughtered ewes in breeding sea...