Dog rose (Rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. It can be propagated under in vitro conditions. After removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on Murashige and Skoog medium (MS ...

A high throughput regeneration protocol has been developed for Lagerstroemia speciosa through node explants under the regime of various plant growth regulators (PGRs). This can provide an alternative mode to seed-grown plants and minimize cost–time regeneration, significantly. Murashige Skoog (MS) medium containing combinations PGRs exhibited a marked stimulatory effect on morphogenesis. Of tri...

Anthers of Nicotiana tabacum cultivar Wisconsin 38 were treated with the mutagen ethyl methane sulfonate and cultured to obtain populations of haploid plants of high genetic variability. The selection of these populations by their photosynthetic efficiency was carried out in a hydroponic culture chamber with a CO(2) atmosphere concentration close to the compensation point. Plants that survived ...

Background: Rosa centifoliais commercially propagated by asexual means but in vitro propagation ensure the production of disease free and healthy plants and browning of explants creates hurdle in their multiplication. Objectives: The aim was to reduce oxidative browning of shoots of R. centifolia in MS medium during in vitro propagation. Materials and Methods: Axillary buds of R. centifolia we...

Physalis peruviana L. is one of the most favorable tropical fruit due to its fast growth and nutritional properties. The current research outlined response drought stress golden berry plants inoculated with arbuscular mycorrhizal fungi Claroideoglomus claroideum propagated in vitro as well from seeds. implementation symbiosis was determined by root colonization, glomalin content, alkaline acid ...

The establishment of new in vitro cultures is often a difficult task due to low growth of initial explants. The explant has to adapt to the new nutritional and environmental conditions, in addition to the surface-disinfection process. Since the explant origin plays an important role, in this work, the effect of the origin of the explants (micropropagated or conventionally propagated plants) in ...

Before acclimatization of Bowiea volubilis, in vitro propagated bulbs were cleaned and separated into four sizes (from 6 to 15 mm). We formed a total 10 groups with 30-30 individuals, distributing the evenly. Four types substrate (peat, perlite, coconut fiber, sand) their mixtures 50-50% used. Previously we examined three parameters: length, diameter weight bulbs; later successfully acclimatize...