Clinical and experimental data have documented the ability of nontransmural myocardial infarction to produce abnormal Q waves on both the epicardial and body surfaces. We undertook this study to define the anatomic determinants of such Q wave development. Thirty dogs were studied before and after occlusion-reperfusion (26 dogs) or latex embolization (four dogs) of the left circumflex coronary a...

A new catheter has been developed which uses diamonds embedded in the tip to enable temperature-guided ablation. The degree subsequent Magnetic Resonance (MR) findings correlate with actual tissue pathology never assessed. To evaluate correlation between MR imaging and lesions assessed by Gross pathology. 20 canines underwent evaluation, after 4 were studied acutely 4-6 hours ablation, 8 animal...

RATIONALE We previously reported that type VI collagen deposition increases in the infarcted myocardium in vivo. To date, a specific role for this nonfibrillar collagen has not been explored in the setting of myocardial infarction (MI). OBJECTIVE To determine whether deletion of type VI collagen in an in vivo model of post-MI wound healing would alter cardiac function and remodeling in the da...

Rationale: We previously reported that type VI collagen deposition increases in the infarcted myocardium in vivo. To date, a specific role for this nonfibrillar collagen has not been explored in the setting of myocardial infarction (MI). Objective: To determine whether deletion of type VI collagen in an in vivo model of post-MI wound healing would alter cardiac function and remodeling in the da...

Fetal striatal cell suspensions were grafted in infarcted striata of adult (300-400 g) rats after occlusion of the middle cerebral artery (MCA). The survival/development of grafted cells, GABA release, GABAA receptor binding and behavioral recovery were investigated. Under halothane anesthesia a silicon-coated plug was inserted intraluminally from the external artery to the brain via the caroti...

Nerve growth factor (NGF) is involved in nerve sprouting, hyper-innervation, angiogenesis, anti-apoptosis, and preservation of cardiac function after myocardial infarction (MI). Positively modulating NGF expression may represent a novel pharmacological strategy to improve post-infarction prognosis. In this study, lentivirus encoding NGF short interfering RNA (siRNA) was prepared, and MI was mod...

The aim of the present study was to evaluate the possible beneficial effects of implantation of laser-irradiated mesenchymal stem cells (MSCs) into the infarcted rat heart. MSCs were isolated from rat bone marrow and grown in culture. The cells were laser irradiated with Ga-Al-As laser (810 nm wavelength), labeled with 5-Bromo-2’deoxyuridine (BrdU), and then implanted into infarcted rat hearts....

AIMS The objective of this study was to investigate whether vascular endothelial growth factor (VEGF) secreted by mesenchymal stem cells (MSC) improves myocardial survival and the engraftment of implanted MSC in infarcted hearts and promotes recruitment of stem cells through paracrine release of myocardial stromal cell-derived factor-1α (SDF-1α). METHODS AND RESULTS VEGF-expressing MSC ((VEGF...