To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI Gm6ATC motif common in the genomic DNA of many bacteria including γ-proteobacteria. Using synthetic ge...

Before 1960, the brain was considered by scientists to be immutable, subject only to genetic control. In the early sixties, however, investigators were seriously speculating that environmental influences might be capable of altering brain structure. By 1964, two research laboratories proved that the morphology and chemistry or physiology of the brain could be experientially altered (Bennett et ...

Anthropogenic nitrogen (N) enrichment of many ecosystems throughout the globe has important ramifications for plant communities. Observational and experimental studies frequently find species richness declines with N enrichment, in concert with increasing primary production. Nitrogen enrichment also reorders species composition, including species turnover through gains and losses of species, ch...

Before 1960, the brain was considered by scientists to be immutable, subject only to genetic control. In the early sixties, however, investigators were seriously speculating that environmental influences might be capable of altering brain structure. By 1964, two research laboratories proved that the morphology and chemistry or physiology of the brain could be experientially altered (Bennett et ...

in order to evaluate the effect of substrate and enrichment material on biological efficiency (be) and other physical and chemical propertiese of medicinal mushroom shiitake (lentinula edodes) an investigation was perfomed as factorial experiment in completely randomized designe with four replications in khorramabad city of lorestan continent iran. experimental factors were substrate in three l...

iron deficiency anemia is one of the nutritional problems of the low income, undernourished populations of the world. in iran, many rural and low socioeconomic urban groups suffer from iron deficiency anemia because of a low iron intake and the low level of public health. to overcome and to prevent iron deficiency, a program was suggested to enrich the bread with iron, calcium and some of the b...

To clarify the organic matter (OM) enrichment of Lishui Sag, factors influencing variable abundance OM in Lingfeng Formation are studied using geochemical data. The source rocks have medium–high total carbon (TOC) values (0.53–3.56%). main type kerogen is II2-III. Compared to shallow marine subfacies rocks, TOC delta front higher. distribution biomarkers shows that redox environment rock sub-ox...

A positive Priming Effect (PE) is defined as an acceleration of the decomposition soil organic matter (SOM) by a fresh (FOM) input. But in literature many studies present this phenomenon extra loss carbon from SOM to atmosphere. actually mixture pools with different turnover rates, and microorganisms generating PE are heterotrophic. Therefore, whether fact C depends on targeted pool (high or lo...

objective(s):  in our study, graphene oxide-tio2 nanocomposite (go/tio2) was prepared and used for the enrichment of rutin from real samples for the first time.  materials and methods:   the synthesized go/tio2 was characterized by x-ray diffraction, scanning electron microscopy, and ft-ir spectra.  the enrichment process is fast and highly efficient. the factors including contact time, ph, and...