In June 2020, many plants exhibited symptoms of root rot and foliar blight in the experimental field common sage Şanlıurfa province, Turkey. The pathogen was identified as Neoscytalidium novaehollandiae based on morphological characteristics phylogenetic analysis partial sequence transcription elongation factor 1-α gene internal transcribed spacer rDNA. Koch’s postulates were fulfilled by succe...

sooty blotch and flyspeck occur on fruit surfaces and result in economic losses due to less attractive appearance. sooty blotch fungi form dark mycelial mats whereas flyspeck fungal agents are well characterized with black, sclerotium-like bodies on fruit surface. whilst more than 60 species have been reported in association with these two fungal diseases, thus far they have not been studied in...

In May 2019, mature jackfruit (Artocarpus heterophyllus) fruits were found infected with fan-shaped white mycelia and brown to black sclerotia at the Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh. The fungus was successfully isolated on potato dextrose agar medium. internal transcribed spacer region of rDNA amplified using primer pair ITS1 ITS4. Based morphology molecul...

Nine Dictyuchus isolates were obtained from floating and decaying twigs and leaves in Anzali lagoon, Anzali County, Guilan province, Iran. They are interestingly distinguished from current Dictyuchus species by the presence of dictyoid and achlyoid type of zoospore discharge, abundant fusiform and spherical gemma, and the absence of any sexual apparatus. In addition, phylogene...

In the present study, a phylogenetic analysis was undertaken based on the internal transcribed spacer (ITS) rDNA and partial β-tubulin gene sequence of the Ganoderma species. The size of the ITS rDNA regions from different Ganoderma species varied from 625 to 673 bp, and those of the partial β-tubulin gene sequence were 419 bp. Based on the results, a phylogenetic tree was prepared which reveal...

Molecular biology methods offer new opportunities to differentiate, identify and type bacterial species and strains. These methods use the variability of nucleic sequences of genes such as 16S rDNA, beta subunit RNA-ase (rpoB), gyrase (gyrB), rDNA internal transcribed spacer and other genes. The aim of this paper is to provide comprehensive information about the methods available to differentia...