A previous study has established that the protein to phospholipid ratio of the intracytoplasmic membranes of Rhodopseudomonas sphaeroides undergoes cyclical fluctuations during synchronous growth of this organism (Lueking, D. R., Fraley, R. T., and Kaplan, S. (1978) J. Biol. Chem. 253, 451-457). This pattern has been shown to result from large changes in the rate of phospholipid synthesis which...

BACKGROUND In its 1993 report the Canadian Royal Commission on New Reproductive Technologies challenged the effectiveness of in vitro fertilization for severe male infertility. To address the Commission's concern, the authors compared the relative effectiveness of in vitro fertilization combined with intracytoplasmic sperm injection for severe male infertility and conventional in vitro fertiliz...

A rapid method for the isolation of intracytoplasmic A particles, the putative intracellular nucleoprotein cores of mouse mammary tumor virus (MTV), is presented. Spontaneous C3H/He mouse mammary tumors and transplantable mouse Leydig cell tumor were used as source material. Large aggregations of intracytoplasmic A particles were separated from cellular contaminants on discontinuous sucrose gra...

A primary culture of undigested fat tissue fragments was used to obtain fat cells in vitro. On day 2 of culture, immature fat cells, which are fibroblast-like fat cells containing fine lipid droplets, appeared, surrounding the fat tissue fragments, and began to proliferate extensively. Afterwards, these fibroblast-like fat cells grew to become multilocular fat cells containing larger intracytop...

The ultrastructure of sectioned cells of mutant and wild type Rhodopseudomonas spheroides has been examined by electron microscopy. The characteristic vesicles associated with the presence of bacteriochlorophyll were found in wild type cells grown with low aeration. These were also found in mutant TA-R which forms bacteriochlorophyll under high aeration. None of the mutants with blocks in bacte...

Intracytoplasmic sperm injection (ICSI) is used to fertilize oocytes by delivering spermatozoa directly into the ooplasm using micromaniplating devices. Mammalian ICSI, was initially designed to examine the fertilization steps in ooplasm.1 ICSI has wide clinical application and become the method of choice to help overcome male infertility, however, the survival rates and fertilization rates of ...

the main role of cumulus cells (cc) is to provide nutritious materials for developing oocytes. follicular fluid (ff) contains the enzyme acid phosphatase, which plays a role in ovulation and fertilization of oocyte. the objective of this prospective study was to evaluate the levels of acid phosphatase in ff and the rate of apoptosis in cc of fertilized and unfertilized oocytes in intracytoplasm...