For most biophysical techniques, characterization of protein-protein interactions is challenging; this is especially true with methods that rely on a physical phenomenon that is common to both of the interacting proteins. Thus, for example, in IR spectroscopy, the carbonyl vibration (1600-1700 cm(-1)) associated with the amide bonds from both of the interacting proteins will overlap extensively...

epoxyalcohols are useful intermediates in complex organic synthesis, and recently they have been recognized as versatile intermediates for synthesis of natural products. the treatments of cis and trans 2-(1-cyclohexenyl) cyclohexanol, separately with paranirto perbenzoic acid gave the corresponding 2-(1- cyclohexenyl) cyclohexanol oxide. on the other hand, 2-cyclohexylidene cyclohexanol oxide w...

Surface analysis: two-dimensional infrared spectroscopy (2D-IR) was used to probe amide and carboxylate functional groups in the vicinity of gold nanoparticles and their aggregates. The data presented shows that the unique ability of 2D-IR spectroscopy to characterize broadening mechanisms and cross peaks offers new insights into gold nanoparticle capping layer structure and dynamics.

Atomic force microscopy-based infrared spectroscopy (AFM-IR) is a rapidly emerging technique that provides chemical analysis and compositional mapping with spatial resolution far below conventional optical diffraction limits. AFM-IR works by using the tip of an AFM probe to locally detect thermal expansion in a sample resulting from absorption of infrared radiation. AFM-IR thus can provide the ...

In the increasing research field of 2D materials such as graphene, molybdenum disulfide MoS2 has attracted great interest due to the existence of a direct bandgap in monolayer MoS2, which gives the possibility of achieving MoS2 fieldeffect transistors or optoelectronic devices. We analyzed by THz time-domain spectroscopy (THz-TDS) up to 2 THz and infrared (IR) spectroscopy, CVD-obtained MoS2 us...

Time-resolved infrared spectroscopy has been proven to be a powerful tool for investigating the structure, dynamics and reactivity of electronically-excited states of inorganic molecules. As applications drive the production of ever more complex molecules however, experimental tools that can deliver more detailed spectroscopic information, or separate multiple contributions to complex signals w...

The molecular spectroscopy of a solution particle by structure resonance modulation spectroscopy is discussed [S. Arnold and A. B. Pluchino, "Infrared Spectrum of a Single Aerosol Particle by Photothermal Modulation of Structure Resonances," Appl. Opt. 21, 4194 (1982); S. Arnold et al., "Molecular Spectroscopy of a Single Aerosol Particle," Opt. Lett. 9, 4 (1984)]. Analytical equations are deri...

The capability of 2D IR spectroscopy to elucidate time-evolving structures is enhanced by a programmable mid-IR pulse shaper that greatly improves the ease, speed, and accuracy of data collection. Traditional ways of collecting 2D IR spectra are difficult to implement, cause distorted peak shapes, and result in poor time resolution and/or phase problems. We report on several methods for collect...

Fourier transform infrared (FT-IR) spectroscopy is a physico-chemical method based on measurement of vibration of a molecule excited by IR radiation at a specific wavelength range. FT-IR spectra of bacterial cells can be used to analyze their total composition, including proteins, fatty acids, carbohydrates, nucleic acids, and lipopolysacharides. FT-IR techniques coupled with different chemomet...

The experimental technique and applications of ultrafast two-dimensional infrared (2D IR) vibrational echo spectroscopy are presented. Using ultrashort infrared pulses and optical heterodyne detection to provide phase information, unique information can be obtained about the dynamics, interactions, and structures of molecular systems. The form and time evolution of the 2D IR spectrum permits ex...