The study objectives were to determine (i) if pre-ovulatory luteinizing hormone (LH) surges, undetected in urine by two immunoradiometric assays (IRMA), were detectable by an ultrasensitive immunofluorometric assay (IFMA) and (ii) the influence of creatinine adjustment on the detection and timing of the urinary LH surges. Daily urine specimens were contributed by healthy 25-36 year old voluntee...

An attribute-based signature (ABS) is a special digital signature created using a dynamic set of issued attributes. For instance, a doctor can sign a medical statement with his name, medical license number and medical speciality. These attributes can be verified along with the signature by any verifier with the correct public keys of the respective attribute issuers. This functionality not only...

We describe the first homogeneous, nonradioactive, high-sensitivity assay for human thyrotropin (TSH). The assay is based on particle immunoassay techniques, wherein 800-nm particles form the basis for the immunochemistry, delivery, and the detection technologies, respectively. Our assay also is the first to involve the use of fragmented monoclonal antibodies (to eliminate serum interferences) ...

We evaluated comparatively six commercially available osteocalcin kits: ELSA-OST-NAT IRMA (CIS), ELSA-Osteo IRMA (CIS), Osteocalcin IRMA (Nichols), OSTK-PR RIA (CIS), OSCA Test Osteocalcin RIA (Henning), and Osteocalcin RIA (Nichols). All of them presented acceptable values of variance, sensitivity, and serial dilutions. However, only the ELSA-OST-NAT, ELSA-Osteo, and OSCA Test kits had analyti...

A human specific monoclonal antibody (D5) raised against a Mr 36,000 cytosolic estrogen receptor component (RE) partially purified from human myometrium was used to develop a simple, rapid, and sensitive solid-phase immunoradiometric assay (IRMA) for the reactive antigen in tissue cytosols from breast tumors, myometrium, endometrium, and endometrial carcinomas. The IRMA did not detect antigen i...

Knowing the epitope specificities of our monoclonal antibodies (MCA) to human choriogonadotropin (hCG), we could design three classes of two-site immunoradiometric assays (IRMA): a combinationof two MCA recognizingtwo separate a-epitopes (a-MCA) provides a system (i.e., an a-IRMA) that measures holo-hCG plus free a-subunitsplus follitropin, lutropin, and thyrotropin, whereas a /3-IRMA, consisti...

IRMA is a set of multimedia and software tools implemented to encourage the dissemination of scientific culture. On the basis of web technologies we have developed an environment to help learner approaching some mathematical topics, which become accessible through historical news, simulation and interactive applications. In this paper, we will explore the main design and implementation issues o...

Irma Isordia-Salas, Robin A. Pixley, Fengling Li , Irma Sainz, R Balfour Sartor, Albert Adam and Robert W. Colman. The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA 19140, USA; Center for Gastrointestinal Biology and Disease, Departments of Medicine, Microbiology and Immunology, University of North Carolina, Chapel Hill, NC, 27599, USA; Department...

We identified two monoclonal antibodies that bind spatially distinct epitopes on insulin-like growth factor I (IGF-I). Using these two antibodies, we developed a simultaneous, two-site immunoradiometric assay (IRMA) specific for IGF-I. This IRMA has no detectable cross reactivity with insulin, proinsulin, prolactin, or somatotropin, and less than 2% crossreactivity with IGF-II. The assay respon...

FIRE is used as a black-box algorithm to extract different groups of image features that are later used for training different classifiers in order to predict the IRMA code. Three types of classifiers are built. The first type is a single classifier that predicts the complete IRMA code. The second type is a two level classifier composed of four classifiers that individually predict each axis of...