The isoenzymes of glucose 6-phosphate dehydrogenase (G6PD) and láclate dehydrogenase (LDH) were studied in R3230AC mammary adenocarcinomas and normal mammary glands of Fischer rats at various times during and after the administration of estradiol-17/3. Total activities of G6PD and LDH were elevated in response to treatment with estrogen; however, only certain isoenzyme species were increased b...

The human leukemic cell lines HL60 and K562, were induced to differentiate terminally by chemical agents. The isoenzyme patterns of lactate dehydrogenase (LD) in the cells before and after differentiation were determined electrophoretically on agarose gels. In general, treatment of the leukemic cells with inducers of differentiation resulted in a quantitative shift of the isoenzyme pattern towa...

The total lactic dehydrogenase (LD) content and LD isoenzyme ratios were studied in homogenized rectal mucosa from 31 patients with established non-specific mucosal ulcerative colitis and from 16 normal subjects. The total LD content was found to be significantly increased in patients with active ulcerative colitis when compared with patients with inactive colitis or with normal subjects. There...

Studies of the three human creatine phosphokinase (EC 2.7.3.2) isoenzymes, MM, MB, and BB, show that important differences exist in substrate dependency of the reaction rates. A method was developed to study these properties in which the ATP formed in the reverse reaction was measured by means of firefly luciferase. With substrate conditions at which the isoenzymes showed substantial difference...

Following acute stroke, creatine kinase and other enzymes are released into the cerebrospinal fluid and blood from injured brain tissue. To determine whether regional differences in brain enzyme activity might exist and therefore affect the amount of enzyme released, we quantified the levels of creatine kinase, adenylate kinase, and lactate dehydrogenase in 12 regions of normal canine brain (n ...

Nine Aspergillus niger isolates differing in virulence were assayed for polygalacturonase (PG) content and isoenzyme pattern in spores, liquid cultures and inoculated onion seeds of cultivar ‘Bianca Agostana’. The isolates differed in PG production quantitatively and in terms of isoenzyme pattern. Isolates An1 and An5, characterised by low PG production in liquid culture and low virulence, lack...

introduction: different isolates of human specifies, giardia intestinalis, also known as giardia lambia, have been shown to vary in sensitivity to drug, isoenzyme pattern, virulence and infectivity, antigens and restriction enzyme pattern. material and methods: using system that distinguishes sequence differences in the metabolic enzyme tpi, dna extracted from trophozoite that culture in modifi...