Three isoenzymes of brewers’ yeast phosphoglucose isomerase which can be resolved by column chromatography on DEAE-cellulose (NAKAGAWA, Y., AND NOLTMANN, E. A. (1967) J. Biol. Chem. 242, 47824788) have been subjected to physical and chemical characterization. In contrast to the pseudoisoenzymes of rabbit muscle phosphoglucose isomerase (BLACKEWRN, M. N., CHIRGWIN J., M. JAMJZS, G. T., KEMPE, T....

The isoenzymes of lactate dehydrogenase (EC 1.1.1.27) were separated with excellent resolution in less than 1 h by high-pressure anion-exchange chromatography. These isoenzymes were determined in some tissue extracts and high-activity serum samples by this technique.

The maximum acid phosphatasic activity was detected in peanut seed at the 5th day of germination. At least, two acid phosphatases were purified by successive chromatography separations on DEAE-Sepharose CL-6B, CM-Sepharose CL-6B, Sephacryl S-100 HR, and Phenyl-Sepharose HP to apparent homogeneity from five days old cotyledon of peanut after germination. These isoenzymes, designated peanut cotyl...

The synthesis rates of different myosin isoenzymes in a single muscle, and of the same isoenzymes in different muscles (soleus, masseter and plantaris), were measured. The rate of total protein synthesis was significantly higher in the soleus [greater than 95% slow myosin (SM)] than in the plantaris [greater than 95% fast myosin (FM)]. Two fast isoenzymes, FM2 and FM3, were synthesized at diffe...

The separation of N-acetyl-beta-D-hexosaminidase isoenzymes from human tissues is used in the diagnosis and differential diagnosis of GM(2) gangliosidosis, since in type 1 the A isoenzyme is deficient and in type 2 both the A and B isoenzymes are deficient. Peripheral blood leucocytes are commonly used for these investigations, and the present study demonstrates that, in addition to these two i...

A procedure incorporating the salient features of ion-exchange column chromatography with ion-exchange h.p.l.c. is described for the fractionation and purification to homogeneity of several membrane-bound rat hepatic phenobarbital (PB)-inducible cytochrome P-450 isoenzymes, including the major PB-inducible species. The resolving power of this technique makes it a highly promising tool for the i...

Tissue distribution and kinetic parameters for the four isoenzymes of pyruvate dehydrogenase kinase (PDK1, PDK2, PDK3 and PDK4) identified thus far in mammals were analysed. It appeared that expression of these isoenzymes occurs in a tissue-specific manner. The mRNA for isoenzyme PDK1 was found almost exclusively in rat heart. The mRNA for PDK3 was most abundantly expressed in rat testis. The m...

We have prepared murine monoclonal antibodies against isolated human bone alkaline phosphatase (ALP, EC 3.1.3.1). Hybridoma supernates were separately screened for reactivity against both human liver and bone ALP. Although most antibody-positive hybrids showed similar reactivity against both isoenzymes, one hybridoma produced an antibody that interacted preferentially with liver ALP. This antib...

Myosin is a major component of skeletal muscle and it plays a central role in determining the physiological performance of adult tissue. Developing muscles contain myosin molecules which are different from the adult forms, and these isoenzymes have been found to be characteristic markers of the diverse physiological and pathological states of muscle tissue. The differences between these isoenzy...

A new method for the separation of alkaline phosphatase isoenzymes by means of high performance liquid chromatography (HPLC) is presented. One isoenzyme was identified in homogenate of small intestine, two were identified in bone, and two in liver, and fragment and biliary isoenzymes were identified in bile. Sera from 32 patients with different diseases of the skeletal system or the liver were ...