Radiolabelled chondroitin 4-sulphate was isolated after incubation of rat rib cartilage with N-acetyl-D-[6-3H]galactosamine. After proteolytic digestion of the tissue with either papain or trypsin the released [3H]chondroitin 4-sulphate was added to an isolated perfused rat liver system. Analysis of perfusate after several hours perfusion showed that radiolabelled amino sugars were secreted by ...

A direct study of the isolated rat liver perfused with oxygenated blood containing amino acids and lysine-epsilon-C(14) has yielded facts indicating that the liver synthesizes practically all the plasma fibrinogen, the albumin fraction, and probably more than 80 per cent of the plasma globulin fraction. The response of the isolated perfused liver in protein synthesis is qualitatively and quanti...

The immunochemical technique, first employed in the study of albumin synthesis in chicken liver slices by Peters and Anfmsen (3), has recently been successfully used with rat liver slices by Marsh and Drabkin (4) as well as by Campbell and Stone (5). The present work extends this technique to the problem of the biosynthesis of plasma lipoprotein by rat liver. The liver has been shown to be the ...

t-2 toxin, a trichothecene mycotoxin, is considered to be one of the most toxic compounds that are produced by molds, particularly the fusarium species. fusarium species have been recognized as a great agricultural problem. they occur worldwide on a variety of plant hosts and cereal grains. the aim of this study was to investigate t-2 toxin-induced liver injury using in situ perfused rat liver....

The role of extracellular Ca2+ in toxic liver injury was examined in two in vitro models of the rat liver, the isolated perfused liver and isolated hepatocytes. The toxins t-butylhydroperoxide and carbon tetrachloride, as well as the Ca2+ ionophore, heptafluorodimethyloctanedione (FOD), were employed to induce cellular injury and death. Lipid peroxidation was also measured as the formation of t...

The objective of this study was to clarify the mechanism underlying hepatic uptake of dioscin (diosgenyl 2,4-di-O-a-L-rhamnopyranosyl-p-D-glucopyranoside), an herbal ingredient with antihepatitis activity, in rats and humans. The liver uptake index (LUI) in vivo, perfused rat liver in situ, rat liver slices, isolated rat hepatocytes, and human organic anion-transporting polypeptide (OATP)-trans...

background: alveolar hypoxia induces monophasic pulmonary vasoconstriction in vivo, biphasic vasoconstriction in the isolated pulmonary artery, and controversial responses in the isolated perfused lung. pulmonary vascular responses to sustained alveolar hypoxia have not been addressed in the isolated perfused rat lung. in this study, we investigated the effect of sustained hypoxic ventilation o...

Background: Alveolar hypoxia induces monophasic pulmonary vasoconstriction in vivo, biphasic vasoconstriction in the isolated pulmonary artery, and controversial responses in the isolated perfused lung. Pulmonary vascular responses to sustained alveolar hypoxia have not been addressed in the isolated perfused rat lung. In this study, we investigated the effect of sustained hypoxic ventilation o...