background: to determine prospectively the prevalence of anaerobic and aerobic infection in early onset (during 72 hours of age) neonatal sepsis, in tehran vali-e-asr hospital. methods: among all the live birth, neonates suspecting of having septicemia were investigated for isolation of micro­organisms. culture bottle containing enriched tryptic soy broth was used for standard blood culture sys...

Three different areas -hospital, food-handling establishments and human dwellings, were surveyed for pathogenic gram-negative bacteria carried on the cuticles of cockroaches. Fifty species of bacteria were identified from all cockroaches. Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii and Enterobacter cloacae were the most frequently found. Pathogenic and potentially pathogenic b...

Proteomic analysis on cell envelope proteins from Gram-negative bacteria requires specific isolation techniques. We found that conventional extraction methods such as osmotic shock cause extracts to be heavily contaminated with soluble cytoplasmic proteins. These cytoplasmic protein contaminants constitute the major signal in proteomic analysis and can overwhelm the signals coming from genuine ...

ABSTRACT            Background and Objectives: Halophilic bacteria can grow and survive in environments with a wide range a wide range of salinities. In this study, we aimed to isolate halophilic bacteria from the Meyghan Lake in Arak (Iran) and evaluate their enzymatic activity.            Methods: Samples were taken from four different areas of the lake. Halophilic bacteria were isolated by...

polycyclic aromatic hydrocarbons (pahs) are one of the most common contaminants found in soil. pahs exhibit a significant concern because of their toxicity, carcinogenic, teratogenic and mutagenic properties. using microorganisms for cleaning the pollution of soil can be cheap, effective and can provide environmental immunity. the goal of this study is to isolate and identify phenanthrene degra...

background and objectives: petroleum hydrocarbons are harmful to the environment, human health, and all other living creatures. oil and its byproducts in contact with water block sunshine to phytoplanktons and thus break the food chain and damage the marine food source. this study aims to isolate the crude oil degrading and biosurfactant producing bacteria from the oil contaminated soils of gac...

background: cryptosporidium parvum causes severe gastroenteritis in immunocompromised human and new borne animals. the organism can be transmitted through water. since small number of c. parvum is infectious, the aim of the present study was to develop a chromatography method for the isolation of c. parvum oocyst in samples with limited number of oocysts. methods: antibody was prepared against ...