Keeney et al. (1) provide a graphic example of where isotype controls don’t work and can in fact lead to erroneous estimations of the target subpopulation. We would agree that for the accurate enumeration of CD34 positive stem cells, isotype controls are not always helpful and indeed there are other examples of rare event analysis where the isotype controls don’t work. We recognize therefore th...

Background Rheumatoid factor (RF) is a well-established marker for the diagnosis and classification of rheumatoid arthritis (RA). Most studies have evaluated IgM RF or isotype-nonspecific total tests. Objectives We importance isotype in this study. Methods An international cohort consisting samples from 52 RA patients 57 suffer other rheumatologic deseases were tested IgA, IgG RF. Clinical biol...

Primary diffuse large B-cell lymphomas (DLBCLs) are aggressive tumors accounting for approximately 40% of B-cell malignancies. The immunoglobulin (Ig) variable region genes have undergone rearrangement and are commonly somatically mutated. The majority show intraclonal variation which indicates that somatic mutation has continued after transformation. Typically, cells of DLBCLs express Ig of a ...

The granulocyte-macrophage colony-stimulating factor (GM-CSF) autoantibody (GMAb) is the causative agent underlying autoimmune pulmonary alveolar proteinosis (aPAP). It consists primarily of the IgG isotype. At present, information on other isotypes of the autoantibody is limited. We detected serum the IgM isotype of GMAb (IgM-GMAb) in more than 80% of patients with aPAP and 22% of healthy subj...

We produced anti-idiotype antibodies to antibody to lipid A from Eikenella corrodens. The ALA-1 monoclonal antibody (immunoglobulin M [IgM] isotype), which had already been produced in our laboratory (T. Kato, I. Takazoe, and K. Okuda, Infect. Immun. 57:656-659, 1989), had reacted strongly with lipid A from E. corrodens, Escherichia coli, and Salmonella minnesota. Four anti-idiotype monoclonal ...

938 Human Vaccines & Immunotherapeutics Volume 9 Issue 4 Table 1 in the article incorrectly identifies the isotype of monoclonal antibodies that are used in stability indicating assays that support the Gardasil product line. Corrections to Table 1, revised Feb 2012, correct 2 mAb isotype designation H16.J4 = IgG2a and H18. J4 = IgG2a, see corrected table below. Correction to: Smith JF, et al. H...

the HI assay can be influenced by non-specific serum inhibitors the reactivity of both HI and SN assays strongly depends on the test virus strain used (homologous or heterologous strains) 1 . HI titers show a peak 2-3 weeks after experimental infection. After a maximum of 22 weeks, these titers decrease and become negative, while ELISA still may give positive resultats 3 . Indirect ELISAs seem ...

In this study BALB/c B cell precursors responsive to the T-independent (TI) type 2 (TI-2) antigen, dextran B1355S (DEX), and the T-dependent (TD) derivative, dextran-Limulus hemocyanin (DEX-Hy) were examined for isotype and idiotope expression using the splenic focus assay. The predominant isotype detected in the TI assay was IgM, while IgA was the predominant isotype expressed in the TD assay....