Abstract This study was conducted at the Agriculture College University of Karbala, Iraq to isolate and morphologically molecularly diagnose thirteen Cladosporium isolates collected from tomato plant residues present in desert regions Najaf Karbala provinces, Iraq. We diagnosed obtained by PCR amplification using ITS1 ITS4 universal primer pair followed sequencing. analysis nucleotide sequences...

Degradation of the wild apple trees in fruit forest Tianshan mountain Xinjiang Province, China, has attracted great attention recent years, and pathogens are believed to be an important responsible factor. We observed that Malus sieversii its understory plant, Urtica cannabina, exhibited similar symptoms leaf spot disease, we suspect they caused by same pathogens. DNA sequencing using ITS1 ITS4...

Cassava brown leaf spot surveys were conducted in the main cassava plantation areas of China between 2007 and 2012 in order to understand the distribution of the disease. Cassava plants were damaged by the disease to different degrees in most of the survey sites. Samples were collected and seven strains were isolated from lesions. The mycelium-breaking plus black light induction method was appl...

Mortierella sp. has been known to produce polyunsaturated fatty acids (PUFAs) such as GLA and AA under normal growth medium conditions. Similarly, under the stress condition, this fungus produces EPA and DHA in their mycelial biomass. Among the 67 soil samples screened from the Western Ghats of India, 11 Mortierella isolates showed the presence of omega-6 and omega-3 fatty acid, mainly GLA, AA,...

Phytase play an important role in phytic acid catalysis that act as a food inhibitor in cereals. Here, we isolated high phytase producing isolates NF191 closely related to Aspergillus fumigatus sp. from piggery soil. DNA was isolated from the fungal culture and amplified the ITS region using ITS1 and ITS4 primer using PCR. The 400-900 bp amplicon was gel eluted and subjected to sequencing. The ...

Rhizoctonia solani isolates obtained from common beans (Phaseolus vulgaris L.) were included in an AG4 anastomosis group in accordance with hyphal anastomosis. In the subgrouping of AG4 isolates, PCR-RFLP patterns in the rDNA-ITS were used. After obtaining the genomic DNA belonging to R. solani AG4, an approximately 700 bp amplification product of the ITS1-5.8S-ITS2 region was obtained with PCR...

The increased incidence of fungal infections in the recent past has been attributed to the increase in the number of human immunodeficiency virus-positive and AIDS patients. Early diagnosis of mycoses in patients is crucial for prompt antifungal therapy. Immunological methods of diagnosis have not been found to be satisfactory, and recent research has been diverted to the use of PCR for the sen...

This study was conducted in the Laboratory of Fungus Department Biology / College Science University Qadisiyah to isolate and diagnose some insulation from fungi isolated imported dried fruits (raisins) province, Iraq. The isolations were diagnosed both morphologically microscopically using classification keys confirm appearance microscopic diagnosis polymerase chain reaction(PCR), And determin...

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

Currently, it is accepted that there are three species that were formerly grouped under Candida parapsilosis: C. para- psilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis. In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences in these nosocomial pathogens. An accurate, fast, and economical identification of fungal speci...