An increasing quantity of chickens is being utilized annually in the poultry industry, producing a huge volume of chicken feather waste which presents a high quality supply of keratin. Keratinases possessing high level of keratinolytic activity on insoluble keratin play a crucial role in hydrolyzing chicken feathers. Ever since the discovery of proteolytic ability as well as water solubility of...

Background; Feather waste is generated in large amounts as a by-product of commercial poultry processing. The main component of feather is keratin. The main purpose of this study was to identify Bacillus spp. (the keratinolytic bacteria) that are able to degrade the feather for producing keratin. Methods; Bacillus spp. Were isolated from the waste of poultries located in Miyaneh city. The bact...

Keratinase is a protease which degrades the insoluble protein keratin that largely found in feather waste. Fifty-seven soil samples were collected from and poultry waste dumping sites. All these subjected for enrichment Whole meal medium containing 1% chicken as sole source of Carbon, nitrogen energy with pH 8.0 incubated at 30°C on shaker condition (150 rpm) 7 days. Out 57 flasks only 21 sh...

Keratinase is a potential industrial enzyme for handling keratinaceous solid wastes and also cleaner dehairing alternative to chemical methods in tanneries. The technologies towards circular economy approach are gaining importance leather manufacturing sector. In this study, new bacterial strain Bacillus sp. KLP1, exhibiting high tolerance sodium chloride sulphide, was isolated. This effectivel...

Due to the high levels of pollution load produced in leather industry, many studies are being conducted replace most hazardous chemicals used. One options is use enzymes which biodegradable and considered environmentally friendly. Hides skins contain non-collagenous substances that require specific remove. The aim study was evaluate effectiveness different enzyme formulations unhairing process ...

The objective of this study was to determine the efficacy of a broad-spectrum protease enzyme, PWD-1 keratinase, upon supplementation to corn-soy starter diets on growth performance of broiler chickens. Three experiments were conducted. In each experiment, 1-d-old broiler chicks were randomly assigned to 24 cage pens of eight birds per pen in a completely randomized design of five experimental ...

Keratinase is an important industrial enzyme, but its application performance limited by low activity. A rational design of 5′-UTRs that increases translation efficiency approach to enhance protein expression. Herein, we optimized the 5′-UTR recombinant keratinase KerZ1 expression element secretory activity in Bacillus subtilis WB600 through Spacer design, RBS screening, and sequence simplifica...

Two hundred thirty four fungal strains were isolated by baiting method, for their feather degradation and keratinase producing ability. Fungi were tested on solid milk agar plates and in submerged culture. Maximum clearing zone was made by Chrysosporium indicum (7mm) on solid agar plates. The highest keratinase production was found in case of Acremonium strictum (74.40Unit/mL & 124.72 Unit/ml i...

Keratinases are proteolytic enzymes predominantly active when keratin substrates are available that attack disulfide bridges in the keratin to convert them from complex to simplified forms. Keratinases are essential in preparation of animal nutrients, protein supplements, leather manufacture, textile processing, detergent formulation, feather meal processing for feed and fertilizer, the pharmac...

Abstract Keratinases can specifically degrade keratins, which widely exist in hair, horns, claws and human skin. There is a great interest developing keratinase to manage keratin waste generated by the poultry industry reusing products agriculture, medical treatment feed industries. Degradation of more environmentally friendly sustainable compared with chemical physical methods. However, wild-t...