We prepared in vitro an mRNA transcript coding for the erythroagglutinating subunit of the kidney bean glycoprotein phytohemagglutinin, E-PHA. The mRNA, injected into Xenopus oocytes, synthesized E-PHA carrying two Asn-linked carbohydrate chains, one of which was processed and acquired resistance to endo-beta-N-acetylglucosaminidase H, as occurs in the native bean cells. When the mannose analog...

Ethylene stimulated RNA and protein synthesis in bean (Phaseolus vulgaris L. var. Red Kidney) abscission zone explants prior to abscission. The effect of ethylene on RNA synthesis and abscission was blocked by actinomycin D. Carbon dioxide, which inhibits the effect of ethylene on abscission, also inhibited the influence of ethylene on protein synthesis. An aging period appears to be essential ...

Proteins extracted from the cell walls of Red Kidney bean hypocotyls, tomato stems, and suspension-cultured sycamore cells can completely inhibit the activity of the polygalacturonases (polygalacturonide hydrolases, EC 3.2.1.15) secreted by the fungal plant pathogens Colletotrichum lindemuthianum, Fusarium oxysporum, and Sclerotium rolfsii. The inhibitor of the C. lindemuthianum polygalacturona...

The purpose of this paper was to estimate and to evaluate the hydration curves of three kinds of dry bean varieties (Phaseolus vulgaris L.), besides adjusting the Peleg’s model to the experimental values under several soaking temperatures. Bean seeds of pinto, black and red kidney varieties were soaked in distilled water in the temperatures of 20, 30, 40 and 50 °C. The constant C1 (Peleg rate c...

use of plant growth promoting bacteria (pgpb) and high quality of seed as agricultural inputs play important roles in sustainable crop production. to study the effects of different levels of seed aging and pgpb's application on field emergence, seedling establishment and yield of two red kidney bean cultivars in field conditions, an experiment was carried out as factorial based in a rcbd d...

This research reports the effects of two metabolic groups of bacteria on physiological activity and cognitive function in C57BL/6 mice. In one experiment, the mice on a diet supplemented with red kidney bean for 24 hr were able to complete an eight-arm radial learning maze in a shorter time than mice on a diet without red kidney beans. The median time required for mice to complete the maze when...

A beta-glucan isolated from the mycelial walls of Phytophthora megasperma var. sojae and a glucan purified from yeast extract stimulate the accumulation of phytoalexins in red kidney bean, Phaseolus vulgaris, and stimulate the accumulation of the phytoalexin, rishitin, in potato tubers, Solanum tuberosum. These glucans have previously been shown to be potent elicitors of glyceollin accumulation...

Inclusion of raw kidney bean (Phaseolus vulgaris) proteins in the diet for rats was shown to affect the weight of some internal organs. Of these, in addition to the well-known hypertrophy of the pancreas attributable to dietary trypsin inhibitors, the observed atrophy of the thymus and the doubling in weight of the small intestine are related to the protein or lectin content of the bean diet, o...

Rats fed a bean diet develop a significant hypocholesterolemia. The catabolism of low density lipoprotein (LDL; d 1.019-1.063 g/ml) was studied in vivo and in vitro in the isolated perfused liver of rats fed either a casein or a bean diet. The clearance of LDL was significantly increased by 100% from 0.38 +/- 0.04 to 0.63 +/- 0.04 ml/h x 100 g body wt in vivo in the bean-fed rat. Similarly, the...