Article Type: Full Length Research Article Polymerase chain reaction (PCR) and loop mediated isothermal amplification (LAMP) represent a major advance in diagnostic methods in terms of speed and sensitivity. However, standard methods for sample preparation prior to PCR and LAMP are laborious and time consuming. Three template preparation methods (crude template, boiling, and commercial DNA extr...

The new rhodium(I) complexes [Rh(Cl)(COD)(R-NHC-(CH2)3Si(O Pr3)3)] (R = 2,6-diisopropylphenyl (2a); n-butyl (2b)) have been synthesised and fully characterised. The study of their application as ketone hydrosilylation catalysts showed a clear N-substituent effect, 2a being the most active catalyst precursor. Complex 2a has been immobilised in the mesoporous materials MCM-41 and KIT-6. The new h...

Opisthorchiasis and cholangiocarcinoma (CCA) are major public health problems in Thailand and countries in the lower Mekong Subregion. Elimination of opisthorchiasis will be an important step toward the prevention, control and reduction of CCA. In order to achieve this goal, a sensitive and robust diagnostic method is required to identify people with current Opisthorchis viverrini sensu lato in...

OBJECTIVE 25-hydroxyvitamin D2/D3 (25-OHD2/D3) determination is a reliable biomarker for vitamin D status. Liquid chromatography-tandem mass spectrometry was recently proposed as a reference method for vitamin D status evaluation. The aim of this work is to compare two commercial kits (Chromsystems and PerkinElmer) for 25-OHD2/D3 determination by our entry level LC-MS/MS. DESIGN AND METHODS C...

In this paper the Macromedia MX software application for training and lectures preparation is presented. On the basis of earlier experiences short characteristics and new possibilities of this tool kit are also discussed. Macromedia MX software is used all over the word coming up to current standards and specifications.

Galway, Ireland Preparation of Microvascular Endothelial Cells for Cultivation with Embryonic Stem CellDerived Cardiomyocytes S. Schmitteckert, C. Ziegler, M.X. Doss, J. Hescheler, A. Sachinidis, A. Rolletschek [email protected] Institute for Biological Interfaces, KIT Campus North, P.O.B. 3640, D-76021 Karlsruhe Center of Physiology and Pathophysiology, Institute of Neurophysiolog...

radiolabeled microspheres of human serum albumin (msa) is of great importance in the analysis of regional lung perfusion. preparation of msa kit has been installed in our division recently is carried out in two stages:1. production of albumin sphere particles with suitable sizes by using the spinning disk technique.2. formulation of msa as a radiopharmacutical kit. so it can be labelled simphy ...