Whole mount in situ hybridization is one of the most widely used techniques in developmental biology. Here, we present a high-resolution double fluorescent in situ hybridization protocol for analyzing the precise expression pattern of a single gene and for determining the overlap of the expression domains of two genes. The protocol is a modified version of the standard in situ hybridization usi...

Fluorescence in situ hybridization (FISH) is a molecular biology technique that enables the localization, quantification, and identification of microorganisms sample. This has found applications several areas, most notably environmental, for quantification diversity assessment and, clinical, rapid diagnostics infectious agents. The FISH method based on fluorescently labeled nucleic acid probe w...

objective: electroporation is the most common method used for the transfection of dna. although this method has been attributed for various cell using different buffer compositions, the effects of dna concentration on the transfection efficiency has not yet been studied. here the correlation between the efficiency of electroporation reaction and increments of dna concentration has been investig...

background: parental anxiety while waiting for the results of amniocentesis has been investigated by many authors. it seems that the implementation of faster techniques such as fluorescence in-situ hybridization (fish) will have some benefits in reducing this anxiety. besides the patients' attitudes to choosing this method, gynecologists who are the persons responsible for treatment, must feel ...

Visualization of chromosome dynamics allows the investigation of spatiotemporal chromatin organization and its role in gene regulation and other cellular processes. However, current approaches to label multiple genomic loci in live cells have a fundamental limitation in the number of loci that can be labeled and uniquely identified. Here we describe an approach we call "track first and identify...