Cryptosporidium spp is now widely accepted as a cause of gastroenteritis. Various methods have been applied to detect oocysts in faeces, but the difficulties of discriminating between non-cryptosporidial bodies, acid fast bodies like cryptosporidia, and cryptosporidia remain. A simple examination in two stages, suitable for routine use is described, using auramine phenol and carbol fuchsine for...

dcchiavello's method of staining.?The refer^Ce is in the book on Virus Diseases of Man y Van Rooyan & Rhodes, 2nd edition 1948, |>age 33 yan Rooyan & Rhodes 1948 could be r'icketted after the words Macchiavello's Method of staining. In the reference at the ^ this reference should be included. Complement fixation test.?The test is ')erforined to detect unknown antigen by a . n?wn antibody. The m...

493 instances of real data being used to describe and expand on discussion of the techniques. Many of the chapters deal with the philosophy of the technique, the advantages and disadvantages associated with it, and the type of empirical questions that can be addressed by the method. Chapters are also adequately referenced to allow further study, if desired, at a greater level of expertise on an...

The detection of Entamoeba histolytica, the causative agent of amebiasis, is an important goal of the clinical microbiology laboratory. To assess the scope of E. histolytica infection, it is necessary to utilize accurate diagnostic tools. As more is discovered about the molecular and cell biology of E. histolytica, there is great potential for further understanding the pathogenesis of amebiasis...

The emergence of new viral infections of man requires the development of robust diagnostic tests that can be applied in the differential diagnosis of acute illness, or to determine past exposure, so as to establish the true burden of disease. Since the recognition in April 2003 of the severe acute respiratory syndrome coronavirus (SARS-CoV) as the causative agent of severe acute respiratory syn...

R apid and accurate diagnosis is a key to the success of an effective treatment of malaria. The global impact of malaria has spurred interest in developing diagnostic strategies that will be effective not only in resource-limited areas but also in developed countries, where expertise in malaria diagnosis is often lacking. Accurate diagnosis of malaria is necessary to prevent morbidity and morta...

The virologic test results of 415 patients with severe acute respiratory syndrome (SARS) were examined. The peak detection rate for SARS-associated coronavirus occurred at week 2 after illness onset for respiratory specimens, at weeks 2 to 3 for stool or rectal swab specimens, and at week 4 for urine specimens. The latest stool sample that was positive by reverse transcription-polymerase chain ...

Meningitis is an infection of the membranes (meninges) surrounding the brain and spinal cord. Meningitis is usually of multiple etiology-bacterial, fungal or viral yet bacteria remain the common etiological agent (Reid & Fallon, 1992). Meningitis can be acute, with a quick onset of symptoms, or chronic, lasting a month or more, or can be mild or aseptic, but the emphasis should be on identifica...

Hemoglobinopathies include qualitative and quantitative disorders of globin synthesis [abnormal hemoglobins (Hbs) and thalassemias, respectively] and comprise the most common inherited disorders of man worldwide. They occur in particularly high frequency in many of the developing countries as a result of selection by endemic malaria, where they can pose a significant public health problem [1]. ...